UTERINE OXYTOCIN RECEPTORS IN AN AUSTRALIAN MARSUPIAL, THE BRUSHTAIL POSSUM, TRICHOSURUS-VULPECULA

被引：12

作者：

SERNIA, C ^{[1
]}

GARCIAARAGON, J ^{[1
]}

THOMAS, WG ^{[1
]}

GEMMELL, RT ^{[1
]}

机构：

[1] UNIV QUEENSLAND,DEPT ANAT,ST LUCIA,QLD 4067,AUSTRALIA

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-PHYSIOLOGY | 1990年 / 95卷 / 01期

关键词：

D O I：

10.1016/0300-9629(90)90021-J

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. 1. Oxytocin receptors in the uterus of the brushtail possum (T. vulpecula) were characterized by radioreceptor assay and compared with those of the sheep and rat uterus. 2. 2. A single oxytocin binding site was found with an affinity (Kd) and receptor concentration (Ro) of 3.0 ± 0.8 nmol/1 and 200 ± 60 fmol/mg protein, respectively (SEM; n = 5). The receptor was stable at -20°C; divalent ions were required for optimum binding. 3. 3. Competitive displacement curves with related peptides showed the following order of specificity: vasotocin > oxytoein > mesotocin = arginine-vasopressin = [Thr4, Gly7-oxytocin > lysine-vasopressin = isotocin >[d(CH2)5, D-Phe2, Ile4, Ala9-NH2]-AVP. 4. 4. It was concluded that oxytocin receptors in the possum have similar characteristics to those of placental mammals. © 1989.

引用

页码：135 / 138

页数：4

共 19 条

[11] NEUROHYPOPHYSEAL PEPTIDES - DESIGN OF TISSUE-SPECIFIC AGONISTS AND ANTAGONISTS [J].