IN-VIVO DETECTION OF ANTHRALIN-DERIVED FREE-RADICALS IN THE SKIN OF HAIRLESS MICE BY LOW-FREQUENCY ELECTRON-PARAMAGNETIC-RESONANCE SPECTROSCOPY

被引:36
作者
MADER, K [1 ]
BACIC, G [1 ]
SWARTZ, HM [1 ]
机构
[1] DARTMOUTH COLL SCH MED, DEPT RADIOL, HANOVER, NH 03755 USA
关键词
DITHRANOL; ESR; EPR; ELECTRON SPIN RESONANCE;
D O I
10.1111/1523-1747.ep12605998
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
Free radicals were directly detected in vivo in the skin of hairless mice by low-frequency electron paramagnetic resonance spectroscopy after topical application of anthralin under pertinent therapeutic conditions. The electron paramagnetic resonance signal intensity increased steadily, reaching a maximum after about 1 d and decreased slowly in the following days, probably because of desquamation of the skin. We conclude from the spectroscopic features (single line with a line width of 6 gauss; g = 2.0036) and from the pharmacokinetic pattern that the observed signal arises from the final products of anthralin metabolism (ether-insoluble polymeric structures- ''anthralin brown''). Two potential antioxidants, vitamin E and the spin trap tert-butylphenylnitrone, decreased the amount of the anthralin-derived radical that was formed. Neither vitamin E radicals nor tert-butylphenylnitrone spin adducts were observed. We suggest that electron paramagnetic resonance is a valuable tool for the noninvasive and direct in vivo monitoring of drug-induced radical formation in the skin under therapeutic conditions.
引用
收藏
页码:514 / 517
页数:4
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