IN-VIVO DETECTION OF ANTHRALIN-DERIVED FREE-RADICALS IN THE SKIN OF HAIRLESS MICE BY LOW-FREQUENCY ELECTRON-PARAMAGNETIC-RESONANCE SPECTROSCOPY

被引:36
作者
MADER, K [1 ]
BACIC, G [1 ]
SWARTZ, HM [1 ]
机构
[1] DARTMOUTH COLL SCH MED, DEPT RADIOL, HANOVER, NH 03755 USA
关键词
DITHRANOL; ESR; EPR; ELECTRON SPIN RESONANCE;
D O I
10.1111/1523-1747.ep12605998
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
Free radicals were directly detected in vivo in the skin of hairless mice by low-frequency electron paramagnetic resonance spectroscopy after topical application of anthralin under pertinent therapeutic conditions. The electron paramagnetic resonance signal intensity increased steadily, reaching a maximum after about 1 d and decreased slowly in the following days, probably because of desquamation of the skin. We conclude from the spectroscopic features (single line with a line width of 6 gauss; g = 2.0036) and from the pharmacokinetic pattern that the observed signal arises from the final products of anthralin metabolism (ether-insoluble polymeric structures- ''anthralin brown''). Two potential antioxidants, vitamin E and the spin trap tert-butylphenylnitrone, decreased the amount of the anthralin-derived radical that was formed. Neither vitamin E radicals nor tert-butylphenylnitrone spin adducts were observed. We suggest that electron paramagnetic resonance is a valuable tool for the noninvasive and direct in vivo monitoring of drug-induced radical formation in the skin under therapeutic conditions.
引用
收藏
页码:514 / 517
页数:4
相关论文
共 18 条
[11]   THE RELEVANCE OF SECONDARY RADICALS IN THE MODE OF ACTION OF ANTHRALIN [J].
LAMBELET, P ;
DUCRET, F ;
LOLIGER, J ;
MAIGNAN, J ;
REICHERT, U ;
SHROOT, B .
FREE RADICAL BIOLOGY AND MEDICINE, 1990, 9 (03) :183-190
[12]   DETECTION OF FREE-RADICALS IN LIVING MICE AFTER INHALATION OF DTBN BY X-BAND ESR [J].
MADER, K ;
STOSSER, R ;
BORCHERT, HH .
FREE RADICAL BIOLOGY AND MEDICINE, 1993, 14 (03) :339-342
[13]  
Nilges M.J., 1989, PHYS MEDICA, V5, P195
[14]  
SHROOT B, 1986, ARZNEIMITTEL-FORSCH, V36-2, P1253
[15]  
SHROOT B, 1992, ACTA DERM-VENEREOL, P10, DOI 10.2340/000155551721012
[16]  
SHROOT B, 1989, PHARM SKIN, V2, P459
[17]  
SITES H, 1991, OXIDATIVE STRESS OXI
[18]  
SWARTZ HM, 1993, PHYS MEDICA, V9, P41