DETECTION OF FREE-RADICALS IN LIVING MICE AFTER INHALATION OF DTBN BY X-BAND ESR

被引：10

作者：

MADER, K ^{[1
]}

STOSSER, R ^{[1
]}

BORCHERT, HH ^{[1
]}

机构：

[1] HUMBOLDT UNIV,DEPT CHEM,O-1040 BERLIN,GERMANY

来源：

FREE RADICAL BIOLOGY AND MEDICINE | 1993年 / 14卷 / 03期

关键词：

ESR; EPR; FREE RADICALS DETECTION IN LIVING MICE; INVIVO; DTBN DISPOSITION IN MICE; INHALATION OF DTBN;

D O I：

10.1016/0891-5849(93)90030-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new application of X-band electron spin resonance (ESR) is demonstrated for in vivo investigations on living mice. It is the first report on in vivo detection, identification, and localization of a free radical after uptake in the body of a living mammal by inhalation. The volatile di-tert-butyl-nitroxyl (DTBN) has been used as a suitable spin probe. After inhalation of DTBN, a superimposed ESR spectrum consisting of a polar (g = 2.0057; a = 1.65 mT) and a nonpolar paramagnetic species (g = 2.0061; a = 1.51 mT) was detected in mice tails. Collected blood exhibits only the polar spectrum. The decay of signals after removing of the DTBN-soaked swab follows first-order kinetics with k = 0.08 min-1.

引用

页码：339 / 342

页数：4

共 17 条

[1] 3-DIMENSIONAL INVIVO ESR IMAGING IN RATS [J].