EFFECTS OF SERUM CONCENTRATION ON LEVEL OF INTEGRATION OF SIMIAN VIRUS (SV40) GENOME AND TRANSFORMATION FREQUENCY IN SV40-INFECTED CHINESE-HAMSTER CELLS

被引：4

作者：

HIRAI, K ^{[1
]}

DEFENDI, V ^{[1
]}

机构：

[1] NEW YORK UNIV, MED CTR, DEPT PATHOL, 550 1ST AVE, NEW YORK, NY 10016 USA

来源：

VIROLOGY | 1976年 / 69卷 / 01期

关键词：

D O I：

10.1016/0042-6822(76)90209-9

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Treatment of Chinese hamster embryo cultures with different serum concentrations during the 1st 48 h after SV40 infection modified the proportion of cells in DNA synthesis, the amount of SV40-DNA integrated, the proportion of cellular DNA with 5-bromodeoxyuridine substitution in both strands, the proportion of polyploid cells and the transformation frequency. All these parameters increase in a coordinated way as a function of the serum concentration. The increase in the number of cells recruited into DNA synthesis probably facilitates the integration of viral DNA with an increased probability of transformation.

引用

页码：229 / 236

页数：8