USE OF FLUORESCENT INSITU HYBRIDIZATION TO DETECT CHROMOSOMAL REARRANGEMENTS IN SOMATIC-CELL HYBRIDS

被引：9

作者：

GIACCIA, AJ

EVANS, JW

BROWN, JM

机构：

[1] Department of Radiation Oncology, Stanford University School of Medicine, Stanford, California

来源：

GENES CHROMOSOMES & CANCER | 1990年 / 2卷 / 03期

关键词：

D O I：

10.1002/gcc.2870020314

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

In situ hybridization of hamster/human hybrids with biotinylated human genomic DNA has revealed that human chromosomal DNA can integrate into the hamster genome and is not always cytologically detectable. This finding helps to explain why discordancy can arise in gene mapping by failing to recognize small pieces of foreign DNA in the rodent genome. Fluorescent in situ hybridization allows one to locate these fragments in rodent chromosomes visually and possibly to identify their chromosome of origin. Copyright © 1990 Wiley‐Liss, Inc., A Wiley Company

引用

页码：248 / 251

页数：4