POLYMERASE CHAIN-REACTION AMPLIFICATION, CLONING AND SEQUENCING OF VARIANT ESCHERICHIA-COLI SHIGA-LIKE TOXIN TYPE-II OPERONS

被引：49

作者：

PATON, AW

PATON, JC

MANNING, PA

机构：

[1] ADELAIDE CHILDRENS HOSP INC,DEPT MICROBIOL,ADELAIDE,SA 5006,AUSTRALIA

[2] UNIV ADELAIDE,DEPT MICROBIOL & IMMUNOL,ADELAIDE,SA 5000,AUSTRALIA

来源：

MICROBIAL PATHOGENESIS | 1993年 / 15卷 / 01期

关键词：

ESCHERICHIA-COLI; SHIGA-LIKE TOXIN; POLYMERASE CHAIN REACTION (PCR); SEQUENCE ANALYSIS;

D O I：

10.1006/mpat.1993.1058

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The polymerase chain reaction (PCR) was used to amplify approximately 1.5 kb segments of DNA containing complete Shiga-like toxin type II operons from Escherichia coli serotypes OX3:H21 and O111:H—. These fragments were cloned and DNA sequence analysis identified further variations compared with published SLT-II sequences. © 1993 Academic Press.

引用

页码：77 / 82

页数：6

共 24 条

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