MOLECULAR VARIANTS OF BETA(2)-MICROGLOBULIN IN RENAL-INSUFFICIENCY

被引:20
作者
VINCENT, C [1 ]
DENNOROY, L [1 ]
REVILLARD, JP [1 ]
机构
[1] CNRS,SERV CENT ANAL,F-69390 VERNAISON,FRANCE
关键词
D O I
10.1042/bj2980181
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Many patients with renal insufficiency treated by dialysis for more than 10 years have tissue deposits of amyloid material containing polymerized beta(2)-microglobulin (beta(2)m) The mechanisms of beta(2)m polymerization and degradation remain unknown. In biological fluids (serum and urine) from haemodialysis patients and in dialysis fluids from patients treated by chronic ambulatory peritoneal dialysis (CAPD), we have characterized different molecular forms of beta(2)m, including proteolytic split products. beta(2)m isoforms of pI 5.7, 5.3 and 4.5-5.0 were isolated from urine and CAPD fluid. The pI 5.3 beta(2)m, but not the other forms, was recovered both as monomers and as dimers. Such dimers were also detected in serum from patients but not from healthy controls. pI 5.3 and 5.7 beta(2)m isoforms were found to be nearly identical by mass spectrometry and by their amino acid sequences. The amino acid sequence of the 43 N-terminal amino acids of beta(2)m of pI 5.0 showed identity with the corresponding region of pI 5.7 beta(2)m. Fragments recovered from CAPD fluid were similar to proteolytic fragments generated from pure pI 5.7 beta(2)m by incubation in mouse ascitic fluid at acidic pH. Furthermore, pure pI 5.7 beta(2)m was converted into more acidic forms of 12 kDa upon incubation in mouse ascitic fluid at acid pH. beta(2)m dimers found in serum may represent a precursor of amyloid fibrils.
引用
收藏
页码:181 / 187
页数:7
相关论文
共 35 条
[1]   INHIBITION EFFECT OF ROSETTE FORMATION BETWEEN HUMAN LYMPHOCYTES AND SHEEP ERYTHROCYTES BY SPECIFIC HEPTAPEPTIDE ISOLATED FROM UREMIC FLUID AND ITS ANALOGS [J].
ABIKO, T ;
KUMIKAWA, M ;
SEKINO, H .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1979, 86 (04) :945-952
[2]  
ARGILES A, 1992, NEPHROL DIAL TRANSPL, V7, P1106
[3]  
Bardin T, 1986, Nephrol Dial Transplant, V1, P151
[4]   3-DIMENSIONAL STRUCTURE OF BETA-2-MICROGLOBULIN [J].
BECKER, JW ;
REEKE, GN .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (12) :4225-4229
[5]  
CAMPISTROL JM, 1992, AM J PATHOL, V7, P241
[7]   INVITRO FORMATION OF AMYLOID FIBRILS FROM INTACT BETA-2-MICROGLOBULIN [J].
CONNORS, LH ;
SHIRAHAMA, T ;
SKINNER, M ;
FENVES, A ;
COHEN, AS .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1985, 131 (03) :1063-1068
[8]   COMPLETE AMINO-ACID SEQUENCE OF BETA2-MICROGLOBULIN [J].
CUNNINGHAM, BA ;
WANG, JL ;
BERGGARD, I ;
PETERSON, PA .
BIOCHEMISTRY, 1973, 12 (24) :4811-4822
[9]  
DAVEY PG, 1982, CLIN CHEM, V28, P1330
[10]   BETA2-MICROGLOBULIN LOCUS ON HUMAN CHROMOSOME-15 [J].
FABER, HE ;
KUCHERLAPATI, RS ;
POULIK, MD ;
RUDDLE, FH ;
SMITHIES, O .
SOMATIC CELL GENETICS, 1976, 2 (02) :141-153