K+ IONOPHORES INHIBIT NERVE GROWTH FACTOR-INDUCED NEURONAL DIFFERENTIATION IN RAT ADRENAL PHEOCHROMOCYTOMA PC12 CELLS

被引：10

作者：

HARADA, H

MORITA, M

SUKETA, Y

机构：

[1] Department of Environmental Biochemistry and Toxicology, University of Schizuoka, School of Pharmaceutical Science, Shizuoka, 422, Yada

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH | 1994年 / 1220卷 / 03期

关键词：

NEURON DIFFERENTIATION; POTASSIUM ION IONOPHORE; NIGERICIN; NERVE GROWTH FACTOR; VALINOMYCIN; (PHEOCHROMOCYTOMA CELL); (RAT);

D O I：

10.1016/0167-4889(94)90154-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Incubation with a K+/H+ ionophore nigericin attenuated the nerve growth factor (NGF)-induced neurite outgrowth in rat pheochromocytoma PC12 cells. However, a Na+/H+ ionophore monensin and a Ca2+ ionophore A23187 did not inhibited the neurite outgrowth. Nigericin also inhibited the NGF-caused induction of acetylcholinesterase and suppression of cell proliferation. These changes were dependent on the amount of the ionophore added to the culture. In addition, a distinct K+ ionophore, valinomycin, similarly inhibited the NGF-induced neuronal differentiation. These results suggest the presence of the K+ ionophore-sensitive mechanism in the NGF-induced differentiation system in PC12 cells.

引用

页码：310 / 314

页数：5

共 26 条

[1] GENE-TRANSFER AND MOLECULAR-CLONING OF THE HUMAN NGF RECEPTOR [J].

CHAO, MV ;

BOTHWELL, MA ;

ROSS, AH ;

KOPROWSKI, H ;

LANAHAN, AA ;

BUCK, CR ;

SEHGAL, A .

SCIENCE, 1986, 232 (4749) :518-521

[2]

CLARI G, 1991, BIOCHEM BIOPH RES CO, V302, P1021

[3] A NEW AND RAPID COLORIMETRIC DETERMINATION OF ACETYLCHOLINESTERASE ACTIVITY [J].

ELLMAN, GL ;

COURTNEY, KD ;

ANDRES, V ;

FEATHERSTONE, RM .

BIOCHEMICAL PHARMACOLOGY, 1961, 7 (02) :88-&

[4] NERVE GROWTH-FACTOR - BIOCHEMISTRY, SYNTHESIS, AND MECHANISM OF ACTION [J].

GREENE, LA ;

SHOOTER, EM .

ANNUAL REVIEW OF NEUROSCIENCE, 1980, 3 :353-402

[5] ESTABLISHMENT OF A NORADRENERGIC CLONAL LINE OF RAT ADRENAL PHEOCHROMOCYTOMA CELLS WHICH RESPOND TO NERVE GROWTH-FACTOR [J].

GREENE, LA ;

TISCHLER, AS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1976, 73 (07) :2424-2428

[6] INHIBITION OF GROWTH FACTOR-INDUCED DIFFERENTIATION OF PC12 CELLS BY MICROINJECTION OF ANTIBODY TO RAS P21 [J].

HAGAG, N ;

HALEGOUA, S ;

VIOLA, M .

NATURE, 1986, 319 (6055) :680-682

[7] K-252A, A POTENT PROTEIN-KINASE INHIBITOR, BLOCKS NERVE GROWTH-FACTOR INDUCED NEURITE OUTGROWTH AND CHANGES IN THE PHOSPHORYLATION OF PROTEINS IN PC12H CELLS [J].

HASHIMOTO, S .

JOURNAL OF CELL BIOLOGY, 1988, 107 (04) :1531-1539

[8] CALCIUM REGULATION OF GROWTH AND DIFFERENTIATION OF MOUSE EPIDERMAL-CELLS IN CULTURE [J].

HENNINGS, H ;

MICHAEL, D ;

CHENG, C ;

STEINERT, P ;

HOLBROOK, K ;

YUSPA, SH .

CELL, 1980, 19 (01) :245-254

[9]

HOSANG M, 1985, J BIOL CHEM, V260, P655

[10] THE TRK PROTOONCOGENE PRODUCT - A SIGNAL TRANSDUCING RECEPTOR FOR NERVE GROWTH-FACTOR [J].

KAPLAN, DR ;

HEMPSTEAD, BL ;

MARTINZANCA, D ;

CHAO, MV ;

PARADA, LF .

SCIENCE, 1991, 252 (5005) :554-558

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