K+ IONOPHORES INHIBIT NERVE GROWTH FACTOR-INDUCED NEURONAL DIFFERENTIATION IN RAT ADRENAL PHEOCHROMOCYTOMA PC12 CELLS

被引：10

作者：

HARADA, H

MORITA, M

SUKETA, Y

机构：

[1] Department of Environmental Biochemistry and Toxicology, University of Schizuoka, School of Pharmaceutical Science, Shizuoka, 422, Yada

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH | 1994年 / 1220卷 / 03期

关键词：

NEURON DIFFERENTIATION; POTASSIUM ION IONOPHORE; NIGERICIN; NERVE GROWTH FACTOR; VALINOMYCIN; (PHEOCHROMOCYTOMA CELL); (RAT);

D O I：

10.1016/0167-4889(94)90154-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Incubation with a K+/H+ ionophore nigericin attenuated the nerve growth factor (NGF)-induced neurite outgrowth in rat pheochromocytoma PC12 cells. However, a Na+/H+ ionophore monensin and a Ca2+ ionophore A23187 did not inhibited the neurite outgrowth. Nigericin also inhibited the NGF-caused induction of acetylcholinesterase and suppression of cell proliferation. These changes were dependent on the amount of the ionophore added to the culture. In addition, a distinct K+ ionophore, valinomycin, similarly inhibited the NGF-induced neuronal differentiation. These results suggest the presence of the K+ ionophore-sensitive mechanism in the NGF-induced differentiation system in PC12 cells.

引用

页码：310 / 314

页数：5

共 26 条

[21] THE EFFECTS OF NERVE GROWTH-FACTOR ON ACETYLCHOLINESTERASE AND ITS MULTIPLE FORMS IN CULTURES OF RAT PC12-PHEOCHROMOCYTOMA CELLS - INCREASED TOTAL SPECIFIC ACTIVITY AND APPEARANCE OF THE 16-S-MOLECULAR FORM [J].

RIEGER, F ;

SHELANSKI, ML ;

GREENE, LA .

DEVELOPMENTAL BIOLOGY, 1980, 76 (01) :238-243

[22]

RYDEL RE, 1987, J NEUROSCI, V7, P3639

[23] ALTERATIONS IN SURFACE PROPERTIES OF CELLS RESPONSIVE TO NERVE GROWTH-FACTOR [J].

SCHUBERT, D ;

LACORBIERE, M ;

WHITLOCK, C ;

STALLCUP, W .

NATURE, 1978, 273 (5665) :718-723

[24] CALCIUM CURRENT INACTIVATION DURING NERVE-GROWTH-FACTOR-INDUCED DIFFERENTIATION OF PC12 CELLS [J].

STREIT, J ;

LUX, HD .

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1990, 416 (04) :368-374

[25]

SUTTER A, 1979, J BIOL CHEM, V254, P5972

[26]

TENG KK, 1993, J NEUROSCI, V13, P3124

← 1 2 3 →