ANTIBODIES AGAINST OLIGOSACCHARIDES COUPLED TO PROTEINS - CHARACTERIZATION OF CARBOHYDRATE SPECIFICITY BY RADIOIMMUNE ASSAY

被引：39

作者：

ZOPF, DA

TSAI, CM

GINSBURG, V

机构：

[1] National Institute of Arthritis, Metabolism, and Digestive Diseases, National Institutes of Health, Bethesda

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1978年 / 185卷 / 01期

关键词：

D O I：

10.1016/0003-9861(78)90144-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Glycoconjugates prepared by diazotizing β-(p-aminophenyl)ethylamine derivatives of oligosaccharides to the hemp seed protein, edestin, are effective immunogens. Rabbits immunized with the conjugates produce antibodies that bind radiolabeled oligosaccharides. Specificity of binding was determined by comparing inhibitory activities of structurally related oligosaccharides in radioimmunoassay. All of three rabbits immunized with a mannotetraose (Manα1-3Manα1-2Manα1-2Man) conjugate, two of five rabbits immunized with a lacto-N-fucopentaose III (Galβ1-4 [Fucα1-3]GlcN Acβ1-3Galβ1-4Glc) conjugate, and four of five rabbits immunized with a lacto-N-tetraose (Galβ1-3GlcNAcβ1-3Galβ1-4Glc) conjugate developed useful antibodies (i.e., antibodies with Ka {slanted equal to or greater-than} 104 liters mol-1 for the corresponding oligosaccharide). These antibodies can be used to detect specific sugar sequences in complex carbohydrates. © 1978.

引用

页码：61 / 71

页数：11

共 28 条

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