PURIFICATION AND SOME PROPERTIES OF ALPHA-L-ARABINOFURANOSIDASE FROM BACILLUS-SUBTILIS-3-6

被引：33

作者：

KANEKO, S ^{[1
]}

SANO, M ^{[1
]}

KUSAKABE, I ^{[1
]}

机构：

[1] UNIV TSUKUBA,INST APPL BIOCHEM,TSUKUBA,IBARAKI 305,JAPAN

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1994年 / 60卷 / 09期

关键词：

D O I：

10.1128/AEM.60.9.3425-3428.1994

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

alpha-L-Arabinofuranosidase (EC 3.2.1.55) was purified from culture supernatant of Bacillus subtilis 3-6. The enzyme had a molecular weight of 61,000 and displayed maximum activity at pH 7.0 and 60 degrees C. It released arabinose from O-alpha-L-arabinofuranosyl-(1-->3)-O-beta-D-xylopyranosyl-(1-->4)-D-xylopyranose (A(1)X(2),), O-beta-D-xylopyranosyl-(1-->4) -[O-alpha-L-arabinofuranosyl-(1-->3)]-O-beta-D-xylopyransoyl-(1-->4)-D-xylopyranose (A(1)X(3)), and arabinan, but not from O-beta-D-xylopyranosyl-(1-->2)-O-alpha-L-arabinofuranosyl-(1-->3)-O-beta-D-xylopyranosyl- (1--4)-O-beta-D-xylopyranosyl-(1-->4)-D-xylopyranose (A(1)X(4)), arabinoxylan, gum arabic, or arabinogalactan.

引用

页码：3425 / 3428

页数：4

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