MELATONIN PROTECTS HUMAN BLOOD-LYMPHOCYTES FROM RADIATION-INDUCED CHROMOSOME-DAMAGE

被引:105
作者
VIJAYALAXMI [1 ]
REITER, RJ [1 ]
MELTZ, ML [1 ]
机构
[1] UNIV TEXAS,HLTH SCI CTR,DEPT CELLULAR & STRUCT BIOL,SAN ANTONIO,TX 78284
来源
MUTATION RESEARCH LETTERS | 1995年 / 346卷 / 01期
基金
美国国家科学基金会;
关键词
MELATONIN; GAMMA RADIATION; FREE RADICAL; CHROMOSOME DAMAGE;
D O I
10.1016/0165-7992(95)90065-9
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Cells in human peripheral blood were treated in vitro with increasing concentrations of melatonin (0.5 or 1.0 or 2.0 mM) for 20 min at 37 +/- 1 degrees C and then exposed to 150 cGy gamma-radiation from a Cs-137 source. The lymphocytes which were pre-treated with melatonin exhibited a significant and concentration-dependent decrease in the frequency of radiation-induced chromosome damage as compared with the irradiated cells which did not receive the pre-treatment. The extent of the reduction in radiation-induced chromosome damage observed with 2.0 mM melatonin was similar to that found in lymphocytes pre-treated with 1.0 M dimethyl sulfoxide, a known free radical scavenger. Melatonin at 2.0 mM (a 500 X lower concentration) was as effective in decreasing the radiation-induced chromosome damage as dimethyl sulfoxide at 1.0 M. These observations may have implications for human protection against damage due to endogenously produced free radicals and also due to exposure to free radical producing physical and chemical mutagens and carcinogens.
引用
收藏
页码:23 / 31
页数:9
相关论文
共 23 条
[1]   POTENTIAL RADIOPROTECTIVE AGENTS .1. HOMOLOGS OF MELATONIN [J].
BLICKENSTAFF, RT ;
BRANDSTADTER, SM ;
REDDY, S ;
WITT, R .
JOURNAL OF PHARMACEUTICAL SCIENCES, 1994, 83 (02) :216-218
[2]  
Ebadi M., 1984, P1
[3]  
JANIAUD P, 1987, NEUROENDOCRINOL LETT, V9, P311
[4]   MODULATION OF RADIATION-INDUCED CHROMOSOME-ABERRATIONS BY DMSO, AN OH RADICAL SCAVENGER .1. DOSE-RESPONSE STUDIES IN HUMAN-LYMPHOCYTES EXPOSED TO 220 KV X-RAYS [J].
LITTLEFIELD, LG ;
JOINER, EE ;
COLYER, SP ;
SAYER, AM ;
FROME, EL .
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 1988, 53 (06) :875-890
[5]   DISTRIBUTION OF MELATONIN IN MAMMALIAN-TISSUES - THE RELATIVE IMPORTANCE OF NUCLEAR VERSUS CYTOSOLIC LOCALIZATION [J].
MENENDEZPELAEZ, A ;
REITER, RJ .
JOURNAL OF PINEAL RESEARCH, 1993, 15 (02) :59-69
[6]  
MENNENGA K, 1990, ADV PINEAL, V4, P69
[7]   IMMUNOHISTOLOGICAL LOCALIZATION OF MELATONIN IN THE PINEAL-GLAND AND RETINA OF THE RAT [J].
MENNENGA, K ;
UECK, M ;
REITER, RJ .
JOURNAL OF PINEAL RESEARCH, 1991, 10 (03) :159-164
[8]  
MILLER EC, 1983, CANCER RES, V43, P1124
[9]  
OKADA S, 1983, RADIOPROTECTORS ANTI, P339
[10]  
PIERPAOLI W, 1991, ANN NY ACAD SCI, V621, P291