EVIDENCE FOR CALMODULIN-BINDING TO THE CYTOPLASMIC DOMAINS OF 2 C-CAM ISOFORMS

被引：30

作者：

EDLUND, M ^{[1
]}

OBRINK, B ^{[1
]}

机构：

[1] KAROLINSKA INST,MED NOBEL INST,DEPT CELL & MOLEC BIOL,BOX 60400,S-10401 STOCKHOLM 60,SWEDEN

来源：

FEBS LETTERS | 1993年 / 327卷 / 01期

关键词：

ADHESION; CALCIUM; CALMODULIN; C-CAM; IMMUNOGLOBULIN SUPER-FAMILY;

D O I：

10.1016/0014-5793(93)81046-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

C-CAM (cell-CAM 105) is a transmembrane cell adhesion molecule, belonging to the immunoglobulin superfamily. It is expressed in epithelia, vessel endothelia and leukocytes, and mediates intercellular adhesion in rat hepatocytes by homophilic binding. Two major isoforms (C-CAM1 and C-CAM2) that differ in their cytoplasmic domains occur. A previous study demonstrated that C-CAM can bind calmodulin in a Ca2+-dependent manner. In this study we have expressed the cytoplasmic domains of C-CAM1 and C-CAM2 in fusion proteins and measured calmodulin binding by a gel overlay assay, using I-125-labelled calmodulin. Our results indicate that the cytoplasmic domains of both C-CAM1 and C-CAM2 can bind calmodulin.

引用

页码：90 / 94

页数：5

共 19 条

[1] CARCINOEMBRYONIC ANTIGENS - ALTERNATIVE SPLICING ACCOUNTS FOR THE MULTIPLE MESSENGER-RNAS THAT CODE FOR NOVEL MEMBERS OF THE CARCINOEMBRYONIC ANTIGEN FAMILY [J].

BARNETT, TR ;

KRETSCHMER, A ;

AUSTEN, DA ;

GOEBEL, SJ ;

HART, JT ;

ELTING, JJ ;

KAMARCK, ME .

JOURNAL OF CELL BIOLOGY, 1989, 108 (02) :267-276

[2] C-CAM (CELL-CAM-105) IS A CALMODULIN BINDING-PROTEIN [J].

BLIKSTAD, I ;

WIKSTROM, T ;

AURIVILLIUS, M ;

OBRINK, B .

FEBS LETTERS, 1992, 302 (01) :26-30

[3]

Carlin R K, 1980, Ann N Y Acad Sci, V356, P73, DOI 10.1111/j.1749-6632.1980.tb29600.x

[4]

CHEUNG PH, 1993, J BIOL CHEM, V268, P6139

[5] MOLECULAR-CLONING AND EXPRESSION OF A NEW RAT-LIVER CELL-CAM105 ISOFORM - DIFFERENTIAL PHOSPHORYLATION OF ISOFORMS [J].

CULIC, O ;

HUANG, QH ;

FLANAGAN, D ;

HIXSON, D ;

LIN, SH .

BIOCHEMICAL JOURNAL, 1992, 285 :47-53

[6]

EDLUND M, 1993, IN PRESS J BIOCH

[7]

FITZSIMONS DP, 1992, J BIOL CHEM, V267, P23903

[8]

HIXSON DC, 1989, CANCER RES, V49, P6788

[9] CHARACTERIZATION OF THE 110-KDALTON ACTIN-BINDING CALMODULIN-BINDING, AND MEMBRANE-BINDING PROTEIN FROM MICROVILLI OF INTESTINAL EPITHELIAL-CELLS [J].

HOWE, CL ;

MOOSEKER, MS .

JOURNAL OF CELL BIOLOGY, 1983, 97 (04) :974-985

[10] STUDY OF CALMODULIN BINDING TO THE ALTERNATIVELY SPLICED C-TERMINAL DOMAIN OF THE PLASMA-MEMBRANE CA2+ PUMP [J].

KESSLER, F ;

FALCHETTO, R ;

HEIM, R ;

MEILI, R ;

VORHERR, T ;

STREHLER, EE ;

CARAFOLI, E .

BIOCHEMISTRY, 1992, 31 (47) :11785-11792

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