AMINO-ACID RECOVERY VIA MICRODIALYSIS AND PHOTOINDUCED FOCAL CEREBRAL-ISCHEMIA IN BRAIN CORTEX OF RATS

被引：17

作者：

MONTALBETTI, L

ROZZA, A

RIZZO, V

FAVALLI, L

SCAVINI, C

LANZA, E

SAVOLDI, F

RACAGNI, G

SCELSI, R

机构：

[1] UNIV PAVIA,FAC FARM,IST FARMACOL,PAVIA,ITALY

[2] IRCCS,LAB ANAL BIOCHIM CLIN C MONDINO,PAVIA,ITALY

[3] UNIV PAVIA,DIPARTIMENTO PATOL UMANA,PAVIA,ITALY

来源：

NEUROSCIENCE LETTERS | 1995年 / 192卷 / 03期

关键词：

BRAIN; FOCAL ISCHEMIA; TRANSCEREBRAL MICRODIALYSIS; RAT; AMINO-ACIDS; PENUMBRA; APOPTOSIS;

D O I：

10.1016/0304-3940(95)11632-7

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

A photochemical method using the Rose Bengal dye as thrombogenic agent was employed to induce focal cerebral ischemia in frontoparietal cortex of rats. A transcerebral microdialysis probe was used to collect samples from ischemic cortical area. An increase in glutamate (6-fold) and in taurine (4-fold) within the first hour occurred. Neuropathological investigations demonstrate a reproducible damaged area surrounded by a thin peripheral area showing neuronal apoptotic phenomena. The method represents a reproducible model of focal cerebral ischemia with neuropathological aspects superimposable to those characteristic of thrombogenic stroke in man. This method could also be relevant in the study of neurotransmitters during the evolution of ischemia. Furthermore, the presence of apoptotic phenomena in the perilesional halo confirms an ischemic penumbra suggesting the significance of preclinical pharmacological trials.

引用

页码：153 / 156

页数：4

共 27 条

[1]

Agnati, Zoli, Kurosawa, Benfenati, Biagini, Zini, Hallstrom, Ungerstedt, Toffano, Fuxe, A new model of focal brain ischemia based on the intracerebral injection of endothelin-1, Ital. J. Neurol. Sci., pp. 49-53, (1991)

[2]

Baldwin, Snape, Williams, Misra, Jones, Snares, Green, Cross, The role of glutamate and GABA in a rat model of focal cerebral ischaemia: biochemical and pharmacological investigations, Neurodegeneration, 2, pp. 129-138, (1993)

[3]

Baldwin, Williams, Snape, Ferreira, Cross, Green, Attenuation by chlormethiazole administration of the rise in extracellular amino acids following focal ischaemia in the cerebral cortex of the rat, Br. J. Pharmacol., 112, pp. 188-194, (1994)

[4]

Benveniste, Drejer, Schousboe, Diemer, Elevation of extracellular concentrations of glutamate and aspartate in rat hippocampus during transient cerebral ischemia monitored by intracerebral microdialysis, J. Neurochem., 43, pp. 1369-1374, (1984)

[5]

Benveniste, Huttemeier, Microdialysis-theory and application, Progress in Neurobiology, 35, pp. 195-215, (1990)

[6]

Choi, Rothman, The glutamate neurotoxicity in hypoxic-ischemic neuronal death, Annu. Rev. Neurosci., 13, pp. 171-173, (1990)

[7]

Deshpande, Berstedt, Linden, Kalimo, Vieloch, Ultrastructural changes in the hippocampal CA1 region following transient cerebral ischemia: evidence against programmed cell death, Exp. Brain Res., 88, pp. 91-105, (1992)

[8]

Di Chiara, In-vivo brain dialysis of neurotransmitters, Trends Pharmacol. Sci., 11, pp. 116-121, (1990)

[9]

Dietrich, Watson, Busto, Ginsberg, Bethea, Photochemically induced cerebral infarction, Acta Neuropathol., 72, pp. 315-325, (1987)

[10]

Grome, Gojowczyk, Hofmann, Graham, Quantitation of photochemically induced focal cerebral ischemia in the rat, J. Cereb. Blood Flow Metab., 8, pp. 89-95, (1988)

← 1 2 3 →