AMINO-ACID RECOVERY VIA MICRODIALYSIS AND PHOTOINDUCED FOCAL CEREBRAL-ISCHEMIA IN BRAIN CORTEX OF RATS

被引：17

作者：

MONTALBETTI, L

ROZZA, A

RIZZO, V

FAVALLI, L

SCAVINI, C

LANZA, E

SAVOLDI, F

RACAGNI, G

SCELSI, R

机构：

[1] UNIV PAVIA,FAC FARM,IST FARMACOL,PAVIA,ITALY

[2] IRCCS,LAB ANAL BIOCHIM CLIN C MONDINO,PAVIA,ITALY

[3] UNIV PAVIA,DIPARTIMENTO PATOL UMANA,PAVIA,ITALY

来源：

NEUROSCIENCE LETTERS | 1995年 / 192卷 / 03期

关键词：

BRAIN; FOCAL ISCHEMIA; TRANSCEREBRAL MICRODIALYSIS; RAT; AMINO-ACIDS; PENUMBRA; APOPTOSIS;

D O I：

10.1016/0304-3940(95)11632-7

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

A photochemical method using the Rose Bengal dye as thrombogenic agent was employed to induce focal cerebral ischemia in frontoparietal cortex of rats. A transcerebral microdialysis probe was used to collect samples from ischemic cortical area. An increase in glutamate (6-fold) and in taurine (4-fold) within the first hour occurred. Neuropathological investigations demonstrate a reproducible damaged area surrounded by a thin peripheral area showing neuronal apoptotic phenomena. The method represents a reproducible model of focal cerebral ischemia with neuropathological aspects superimposable to those characteristic of thrombogenic stroke in man. This method could also be relevant in the study of neurotransmitters during the evolution of ischemia. Furthermore, the presence of apoptotic phenomena in the perilesional halo confirms an ischemic penumbra suggesting the significance of preclinical pharmacological trials.

引用

页码：153 / 156

页数：4

共 27 条

[11]

Heiss, Graf, The ischemic penumbra, Current Opinion in Neurology, 7, pp. 11-19, (1994)

[12]

Karpiak, Tagliavia, Wakade, Animal model for the study of drug in ischemic stroke, Annu. Rev. Pharmacol. Toxicol., 29, pp. 403-414, (1989)

[13]

Less, Contributory mechanisms in the causation of neurodegenerative disorders, Neuroscience, 54, pp. 287-322, (1993)

[14]

Lyden, Lonzo, Combination therapy protects ischemic brain in rats, Stroke, 25, pp. 189-195, (1994)

[15]

Mac Manus, Hill, Huang, Rasquinha, Buchan, DNA damage consistent with apoptosis in transient focal ischemic neocortex, NeuroReport, 5, pp. 493-496, (1994)

[16]

May, Finck, Sulfated glicoprotein 2: new relationships of this multifunctional protein to neurodegeneration, Trends Neurosci., 15, pp. 391-392, (1992)

[17]

Mehmet, Yue, Squier, Lorek, Cady, Penrice, Sarraf, Wylezinka, Kirkbride, Cooper, Brown, Wyatt, Reynolds, Edwards, Increased apoptosis in the cingulate sulcus of newborn piglets following transient hypoxia-ischemia is relayed to the degree of high energy phosphate depletion during the insult, Neurosci. Lett., 181, pp. 121-125, (1994)

[18]

Mordecai, Globus, Busto, Martinez, Valdes, Dietrich, Ginsberg, Comparative effect of transient global ischemia on extracellular levels of glutamate, glycine and γ-aminobutyric acid in vulnerable and non-vulnerable brain regions in the rat, J. Neurochem., 57, pp. 470-477, (1991)

[19]

Oppenheim, Cell death denying development of the nervous system, Annals Reviews Inc., 14, pp. 455-501, (1991)

[20]

Raff, Social controls on cell survival and cell death, Nature, 356, pp. 397-400, (1992)

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