PRODUCTIVITY AND SOME PROPERTIES OF EGG-YOLK ANTIBODY (IGY) AGAINST HUMAN ROTAVIRUS COMPARED WITH RABBIT IGG

被引：83

作者：

HATTA, H ^{[1
]}

TSUDA, K ^{[1
]}

AKACHI, S ^{[1
]}

KIM, M ^{[1
]}

YAMAMOTO, T ^{[1
]}

机构：

[1] FUKUYAMA UNIV, DEPT BIOTECHNOL, FUKUYAMA, HIROSHIMA 72902, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1993年 / 57卷 / 03期

关键词：

D O I：

10.1271/bbb.57.450

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Productivity and some properties of anti-Human Rotavirus (HRV) hen egg yolk antibody (IgY) were compared with those of anti-HRV rabbit serum antibody (IgG). The hens immunized with HRV (Wa strain, serotype 1 and Mo strain, serotype 3) were found to continuously to lay eggs without any change in the egg laying rate and the yolk of the eggs laid over a year showed a high level of neutralization titer against HRV. The production of anti-HRV IgY by a hen (one year) was at least 15 times (anti-Wa) and 120 times (anti-Mo) more effective than those by an immunized rabbit in the neutralization titer of the antibodies. The stability of anti-HRV IgY at temperature above 70-degrees-C and low pH 2-3 was less than that of anti-HRV rabbit IgG. The temperature corresponding to the maximum of denaturation endotherm (T(max)) of IgY was 73.9-degrees-C while that of rabbit IgG was 77.0-degrees-C in the analysis by differential scanning calorimetry. This discrepancy in heat and acidic pH stability found between the two antibodies as discussed with regard to their protein structures.

引用

页码：450 / 454

页数：5

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