EFFECTS OF RECOMBINANT HUMAN EXTRACELLULAR-SUPEROXIDE DISMUTASE TYPE-C ON MYOCARDIAL REPERFUSION INJURY IN ISOLATED COLD-ARRESTED RAT HEARTS

被引:17
作者
HATORI, N
SJOQUIST, PO
MARKLUND, SL
PEHRSSON, SK
RYDEN, L
机构
[1] KAROLINSKA HOSP, DEPT CARDIOL, DIV THORAC, S-10401 STOCKHOLM 60, SWEDEN
[2] HASSLE RES LABS, MOLNDAL, SWEDEN
[3] UMEA UNIV, DEPT CLIN CHEM, S-90187 UMEA, SWEDEN
关键词
MYOCARDIAL ISCHEMIA; REPERFUSION INJURY; SUPEROXIDE DISMUTASE; RAT HEART; CARDIOPLEGIA; FREE RADICALS;
D O I
10.1016/0891-5849(92)90075-R
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The efficacy of recombinant human extracellular-superoxide dismutase type C (EC-SOD C) on myocardial reperfusion injury was explored in hypothermically arrested rat hearts, as was its site of action. Forty isolated working rat hearts were subjected to 30 min of global ischemia followed by 30 min of reperfusion. The hearts were arrested by the administration of 10 mL of cold perfusate at the onset of ischemia. At the same time, they were randomly assigned to one of five groups; A: cold perfusate only; B: cold perfusate + EC-SOD C 10.4 mg/L (30,000 U/L); C: cold perfusate + bovine CuZn-SOD 7.5 mg/L (30,000 U/L); D: cold perfusate + EC-SOD C 10.4 mg/L + heparin 50,000 U/L; E: cold perfusate + heparin 50,000 U/L. Heparin was given to prevent binding of EC-SOD C to endothelial cell surfaces. Left ventricular function was studied before ischemia and at the end of reperfusion. Percent recovery of maximal left ventricular dP/dt after reperfusion was more pronounced in group B (109 +/- 24%; p < .05) than in groups A (42 +/- 40%), C (47 +/- 36%), D (44 +/- 33%) and E (58 +/- 25%). Likewise, percent recovery of the double product (heart rate x systolic left ventricular pressure) was better in group B (104 +/- 18%; p < .05) than in the other groups (A: 47 +/- 37%, C: 49 +/- 36%, D: 50 +/- 35%, E: 69 +/- 31%). Compared to the preischemic level, creatine kinase increased significantly in the coronary effluent after reperfusion in groups A, C, D, and E, but not in group B. The results suggest that EC-SOD C, which attaches to the endothelial cell surfaces, might be particularly effective as protection against myocardial reperfusion injury when given together with cardioplegic solution.
引用
收藏
页码:137 / 142
页数:6
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