REACTIVE OXYGEN SPECIES ARE INVOLVED IN THE ACTIVATION OF CELLULAR PHOSPHOLIPASE-A2

被引：86

作者：

GOLDMAN, R

FERBER, E

ZORT, U

机构：

[1] WEIZMANN INST SCI,DEPT HORMONE RES,IL-76100 REHOVOT,ISRAEL

[2] MAX PLANCK INST IMMUNBIOL,W-7800 FREIBURG,GERMANY

来源：

FEBS LETTERS | 1992年 / 309卷 / 02期

关键词：

PHOSPHOLIPASE-A2; NADPH OXIDASE; REACTIVE OXYGEN SPECIES; DIPHENYLENE IODONIUM; VANADATE; TYROSINE PROTEIN PHOSPHATASE;

D O I：

10.1016/0014-5793(92)81092-Z

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Vanadate (V) potentiated (4- to 10-fold) the activation of cellular phospholipase A2 (PLA2) induced by H2O2 (H), a phorbol ester (T), a Ca2+-ionophore (A) and opsonized zymosan in macrophages. V+H induced in intact cells the activation and translocation of PLA2 and protein kinase C (PKC) to the plasma membrane. V+H and V+T+A induced strong chemiluminescence (CL) which was abrogated by a specific NADPH oxidase inhibitor diphenylene iodonium (DPI). DPI markedly suppressed the stimulation of PLA2 by V+T+A and V+OZ. The results suggest that the formation of endogenous reactive oxygen species (ROS) is important for PLA2 activation.

引用

页码：190 / 192

页数：3

共 22 条

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