AMINOOXY ANALOGS OF SPERMIDINE EVIDENCE THE DIVERGENT ROLES OF THE CHARGED AMINO NITROGENS IN THE CELLULAR PHYSIOLOGY OF SPERMIDINE

被引:18
作者
HYVONEN, T
KEINANEN, TA
KHOMUTOV, AR
KHOMUTOV, RM
ELORANTA, TO
机构
[1] UNIV KUOPIO,DEPT BIOCHEM & BIOTECHNOL,SF-70211 KUOPIO,FINLAND
[2] RUSSIAN ACAD SCI,AN BAKH BIOCHEM INST,MOSCOW 117071,RUSSIA
[3] RUSSIAN ACAD SCI,VA ENGELHARDT MOLEC BIOL INST,MOSCOW 117984,RUSSIA
关键词
AOEPU; APAPA; POLYAMINE INTERCONVERSIONS; SPERMIDINE PHYSIOLOGY;
D O I
10.1016/0024-3205(94)00958-9
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Two recently devised spermidine analogues, N-[2-aminooxyethyl]-1,4-diaminobutane (AOEPU) and 1-aminooxy-3-N-[3-aminopropyl]-aminopropane (APAPA), were used to elucidate the role of charge distribution in the functions of spermidine in cultured baby hamster kidney cells. The drugs did not affect cell proliferation nor did they relieve the growth-arrest but potentiated the metabolic disturbances caused by DL-alpha-difluorbmethylornithine (DFMO). Neither drug affected spermidine uptake but both competed with putrescine uptake. Neither drug could replace spermidine in the control of S-adenosylmethionine decarboxylase and accumulation of the reaction product. APAPA prevented spermine synthesis and showed that modest putrescine synthesis take place in the presence of DFMO. AOEPU, but not APAPA, interfered with cellular constituents resulting in. enzymatic formation, accumulation and excretion to culture medium of UV-absorbing catabolites.
引用
收藏
页码:349 / 360
页数:12
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