A METHOD FOR THE SIMULTANEOUS DETERMINATION OF VITAMIN-D2, VITAMIN-D3 AND THEIR METABOLITES IN PLASMA AND ITS APPLICATION TO PLASMA SAMPLES OBTAINED FROM NORMAL SUBJECTS AND PATIENTS

被引：13

作者：

MASUDA, S

OKANO, T

KOBAYASHI, T

机构：

[1] Department of Hygienic Sciences, Kobe Women's College of Pharmacy, Kobe

来源：

FOOD CHEMISTRY | 1992年 / 45卷 / 03期

关键词：

D O I：

10.1016/0308-8146(92)90117-K

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A method has been established for the simultaneous determination of vitamins D2,D3 and their metabolites in plasma. Vitamin D2/D3, 25-hydroxyvitamin D2/D3 (25-OH-D2/D3), 24R,25-dihydroxyvitamin D2/D3 [24,25(OH)2D2/D3] and 1-alpha,25-dihydroxyvitamin D2/D3 [1,25(OH)2D2/D3] in 1.5 ml of a plasma sample were simultaneously assayed. The method includes extraction of lipid, followed by three steps of high-performance liquid chromatography (HPLC) for clean-up and separation. Vitamin D2/D3 and 25-OH-D2/D3 were quantified by a UV detector, while 24,25(OH)2D2/D3 and 1,25(OH)2D2/D3 were assayed by competitive protein binding assay (CPBA) and radioreceptor assay (RRA) methods, respectively. Existing interfering substances in a sample could be effectively eliminated by HPLC and reliable results with small coefficients of variation were obtained. The method was applied to plasma samples obtained from normal subjects and patients with epilepsy, liver cirrhosis, leukaemia, diabetes, sensorineural deafness and hypophosphataemic osteomalacia; satisfactory results were obtained.

引用

页码：215 / 225

页数：11

共 27 条

[11]

JONES G, 1985, MODERN CHROMATOGRAPH, P73

[12]

KATSUMI K, 1987, CHEM PHARM BULL, V35, P970

[13]

KOBAYASHI T, 1986, J MICRONUTR ANAL, V2, P1

[14]

KOBAYASHI T, 1983, J NUTR SCI VITAMINOL, V29, P271, DOI 10.3177/jnsv.29.271

[15]

KURODA E, 1981, J NUTR SCI VITAMINOL, V27, P55, DOI 10.3177/jnsv.27.55

[16]

LAMBERT PW, 1981, J LAB CLIN MED, V98, P536

[17] INTERRELATIONSHIPS IN RATS OF TISSUE POOLS OF CHOLECALCIFEROL AND 25-HYDROXYCHOLECALCIFEROL FORMED IN UV-LIGHT [J].

LAWSON, DEM ;

SEDRANI, SH ;

DOUGLAS, J .

BIOCHEMICAL JOURNAL, 1986, 233 (02) :535-540

[18]

LAWSON DEM, 1979, VITAM HORM, V37, P1

[19] LOW CIRCULATING VITAMIN-D IN OBESITY [J].

LIEL, Y ;

ULMER, E ;

SHARY, J ;

HOLLIS, BW ;

BELL, NH .

CALCIFIED TISSUE INTERNATIONAL, 1988, 43 (04) :199-201

[20]

MASUDA S, 1989, J NUTR SCI VITAMINOL, V35, P211, DOI 10.3177/jnsv.35.211

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