INVITRO CLEAVAGE OF HPV16 E6 AND E7 RNA FRAGMENTS BY SYNTHETIC RIBOZYMES AND TRANSCRIBED RIBOZYMES FROM RNA-TRIMMING PLASMIDS

被引：27

作者：

HE, YK ^{[1
]}

LU, CD ^{[1
]}

QI, GR ^{[1
]}

机构：

[1] ACAD SINICA,SHANGHAI INST BIOCHEM,SHANGHAI 200031,PEOPLES R CHINA

来源：

FEBS LETTERS | 1993年 / 322卷 / 01期

关键词：

RIBOZYME; HUMAN PAPILLOMAVIRUS TYPE-16; RNA-TRIMMING PLASMID; INVITRO CLEAVAGE; INVITRO TRANSCRIPTION;

D O I：

10.1016/0014-5793(93)81102-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A RNA-trimming plasmid pRG523 is constructed, in which three Rz genes, GR5(5'-cis-Rz gene), HR2G(trans-Rz gene) and GR3(3'-cis-Rz gene), are arranged in the order from 5' to 3' downstream from the T7 promoter. In vitro transcription of this plasmid shows that the trans-Rz can be trimmed to definite lengths by the cis-Rz on both sides of the trans-Rz. In vitro cleavage of HPV16 E6 and E7 RNA fragments of different lengths by synthetic Rz and that of E7 RNA with a length of 171 nt by synthetic Rz and transcribed Rzs with different lengths of flanking sequences is studied. The results show that the non-base-pairing flanking sequences on both Rz and target RNA can affect the cleavage reaction.

引用

页码：21 / 24

页数：4

共 14 条

[1] BIOLOGICAL CATALYSIS BY RNA [J].

CECH, TR ;

BASS, BL .

ANNUAL REVIEW OF BIOCHEMISTRY, 1986, 55 :599-629

[2]

DESHLER JD, 1992, 6TH FAOB C SHANGH, P23

[3] SIMPLE RNA ENZYMES WITH NEW AND HIGHLY SPECIFIC ENDORIBONUCLEASE ACTIVITIES [J].

HASELOFF, J ;

GERLACH, WL .

NATURE, 1988, 334 (6183) :585-591

[4] CLEAVAGE OF SPECIFIC SITES OF RNA BY DESIGNED RIBOZYMES [J].

KOIZUMI, M ;

IWAI, S ;

OHTSUKA, E .

FEBS LETTERS, 1988, 239 (02) :285-288

[5] INHIBITION OF REPLICATION OF HIV-1 BY RETROVIRAL VECTORS EXPRESSING TAT-ANTISENSE AND ANTI-TAT RIBOZYME RNA [J].

LO, KMS ;

BIASOLO, MA ;

DEHNI, G ;

PALU, G ;

HASELTINE, WA .

VIROLOGY, 1992, 190 (01) :176-183

[6] INVITRO CLEAVAGE OF HIV-1 VIF RNA BY A SYNTHETIC RIBOZYME [J].

LORENTZEN, EU ;

WIELAND, U ;

KUHN, JE ;

BRAUN, RW .

VIRUS GENES, 1991, 5 (01) :17-23

[7]

Sambrook J., 1989, MOL CLONING LAB MANU

[8] RIBOZYMES AS POTENTIAL ANTI-HIV-1 THERAPEUTIC AGENTS [J].

SARVER, N ;

CANTIN, EM ;

CHANG, PS ;

ZAIA, JA ;

LADNE, PA ;

STEPHENS, DA ;

ROSSI, JJ .

SCIENCE, 1990, 247 (4947) :1222-1225

[9] EXPRESSION OF HUMAN PAPILLOMAVIRUS TYPE-16 E7 GENE INDUCES DNA-SYNTHESIS OF RAT 3Y1 CELLS [J].

SATO, H ;

FURUNO, A ;

YOSHIIKE, K .

VIROLOGY, 1989, 168 (01) :195-199

[10] RIBOZYME-MEDIATED CLEAVAGE OF C-FOS MESSENGER-RNA REDUCES GENE-EXPRESSION OF DNA-SYNTHESIS ENZYMES AND METALLOTHIONEIN [J].

SCANLON, KJ ;

JIAO, L ;

FUNATO, T ;

WANG, W ;

TONE, T ;

ROSSI, JJ ;

KASHANISABET, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (23) :10591-10595

← 1 2 →