FULL SEQUENCE AND CHARACTERIZATION OF 2 INSECT DEFENSINS - IMMUNE PEPTIDES FROM THE MOSQUITO AEDES-AEGYPTI

被引：36

作者：

CHALK, R ^{[1
]}

ALBUQUERQUE, CMR ^{[1
]}

HAM, PJ ^{[1
]}

TOWNSON, H ^{[1
]}

机构：

[1] UNIV LIVERPOOL, LIVERPOOL SCH TROP MED, DIV BIOMED SCI, LIVERPOOL L3 5QA, MERSEYSIDE, ENGLAND

来源：

PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES | 1995年 / 261卷 / 1361期

基金：

英国惠康基金;

关键词：

D O I：

10.1098/rspb.1995.0139

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We report the complete amino acid sequence and biological activity of two immune peptides, from the yellow fever mosquito Aedes aegypti, that are induced in response to infection. Both peptides display biological activity against the Gram positive microbe Micrococcus luteus: and substantial sequence homology to insect defensins, small heat-stable, antibiotic peptides previously described from several non-vector insects. These mosquito peptides, designated Ae. aegypti defensins A and B, are isoforms. Defensin B is the most abundant antibacterial peptide in this species whereas defensin A is much less abundant and carries two amino acid substitutions compared to defensin B, making it more basic in character. Apparent convergence between isoforms from Ae. aegypti and the fleshfly Phormia terranovae is discussed. The synergistic :activity previously described between Ae. aegypti immune haemolymph and lysozyme is not caused by these peptides because synergy occurred only at concentrations far outside the physiological range seen in Ae. aegypti.

引用

页码：217 / 221

页数：5

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