METABOLIC LOAD AND HETEROLOGOUS GENE-EXPRESSION

被引：493

作者：

GLICK, BR

机构：

[1] Department of Biology University of Waterloo, Waterloo

来源：

BIOTECHNOLOGY ADVANCES | 1995年 / 13卷 / 02期

关键词：

RECOMBINANT DNA; GENE EXPRESSION; GENETIC ENGINEERING; PROTEIN PRODUCTION; PLASMID STABILITY; HIGH EXPRESSION; FERMENTATION STRATEGIES;

D O I：

10.1016/0734-9750(95)00004-A

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The expression of a foreign protein(s) in a recombinant host cell or organism often utilizes a significant amount of the host cell's resources, removing those resources away from host cell metabolism and placing a metabolic load (metabolic drain, metabolic burden) on the host. As a consequence of the imposed metabolic load, the biochemistry and physiology of the host may be dramatically altered. The numerous physiological changes that may occur often lowers the amount of the target foreign protein that is produced and eventually recovered from the recombinant organism. In this review the physiological changes to host cells, the causes of the phenomenon of metabolic load, and several strategies to avoid some of the problems associated with metabolic load are elaborated and discussed.

引用

页码：247 / 261

页数：15