COMPLETE HYDROLYSIS OF STEROID CONJUGATES AND QUANTITATIVE EXTRACTION OF FREE STEROIDS FROM URINE

被引：15

作者：

VANDENHEUVEL, FA

机构：

来源：

CANADIAN JOURNAL OF BIOCHEMISTRY | 1967年 / 45卷 / 02期

关键词：

D O I：

10.1139/o67-022

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple procedure for the serial hydrolysis and extraction of 5-ml urine samples is described. The samples are incubated at pH 4.45 for 24 hours at 37[degree]C with high concentrations of [beta]-glucuronidase and sulfatases from Helix pomatia. The incubated samples are freeze-dried and the residual solids are repeatedly extracted with a total of 15 ml of chloroform-methanol (1:2, v/v). The overall efficiency is over 99%. A method for the systematic estimation of urinary steroids based on the combined thin-layer and gas-liquid chromatographic analysis of total extracts is outlined.

引用

页码：191 / +

页数：1