STRUCTURE OF HUMAN TYPE-II 5-ALPHA-REDUCTASE GENE

被引：206

作者：

LABRIE, F ^{[1
]}

SUGIMOTO, Y ^{[1
]}

LUUTHE, V ^{[1
]}

SIMARD, J ^{[1
]}

LACHANCE, Y ^{[1
]}

BACHVAROV, D ^{[1
]}

LEBLANC, G ^{[1
]}

DUROCHER, F ^{[1
]}

PAQUET, N ^{[1
]}

机构：

[1] UNIV LAVAL,QUEBEC CITY G1K 7P4,QUEBEC,CANADA

来源：

ENDOCRINOLOGY | 1992年 / 131卷 / 03期

关键词：

D O I：

10.1210/en.131.3.1571

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The best known activity of steroid 5-alpha-reductase is the transformation of testosterone into dihydrotestosterone, the most potent androgen. Two types of human steroid 5-alpha-reductase cDNAs and the type I gene have previously been isolated and characterized. This report describes the isolation and characterization of the human type II 5-alpha-reductase gene, the gene most likely responsible for male pseudohermaphroditism due to 5-alpha-reductase deficiency as well as the one presumed to be involved in major androgen-related diseases such as prostate cancer and benign prostatic hyperplasia. The type II 5-alpha-reductase gene contains five exons of 352, 164, 102, 151 and 1695 bp. respectively, which share 43.8% to 64.1% homology with exons of the corresponding type I gene. These exons are separated by four introns of > 29, and approximately 2.3, 2.0 and 3.0 kb. Analysis of primer extension products by polyacrylamide gel electrophoresis as well as by subcloning and sequencing reveals a start site located 71 nucleotides upstream the ATG initiating codon.

引用

页码：1571 / 1573

页数：3

共 18 条

[1] STRUCTURAL AND BIOCHEMICAL-PROPERTIES OF CLONED AND EXPRESSED HUMAN AND RAT STEROID 5-ALPHA-REDUCTASES [J].