CLONING OF THE CREATININE AMIDOHYDROLASE GENE FROM PSEUDOMONAS SP PS-7

被引：13

作者：

YAMAMOTO, K

OKA, M

KIKUCHI, T

EMI, S

机构：

[1] Tsuruga Institute of Biotechnology, Toyobo Co. Ltd., Tsuruga-shi, Fukui 914

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1995年 / 59卷 / 07期

关键词：

D O I：

10.1271/bbb.59.1331

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The gene coding creatinine amidohydrolase (EC 3.5.2.10) was isolated from Pseudomonas sp, PS-7. The primary structure of creatinine amidohydrolase deduced from the nucleotide sequence showed that the protein is composed of 259 amino acids and has a molecular weight of 28,437. The enzymatic property of creatinine amidohydrolase produced by recombinant E. coli was identical with those by Psendomonas sp. PS-7.

引用

页码：1331 / 1332

页数：2

共 17 条

[11] CREATININE AMIDOHYDROLASE (CREATININASE) FROM PSEUDOMONAS-PUTIDA - PURIFICATION AND SOME PROPERTIES [J].