ELECTRICAL COMMUNICATION OF POLYETHYLENE GLYCOL-MODIFIED GLUCOSE-OXIDASE IN CARBON-PASTE AND ITS APPLICATION TO THE ASSAY OF GLUCOSE

被引：17

作者：

YABUKI, S

MIZUTANI, F

KATSURA, T

机构：

[1] National Institute of Bioscience and Human-Technology, Tsukuba, Ibaraki, 305

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 1993年 / 13卷 / 1-3期

关键词：

D O I：

10.1016/0925-4005(93)85352-B

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An amperometric enzyme electrode was constructed by making use of polyethylene glycol-modified glucose oxidase (PEG-GOD) as a catalyst and carbon paste (CP) as a matrix. The electrode retained an enzyme activity of 20 MU cm-2, which was higher than the CP containing unmodified GOD electrode. Electrical communication, i.e., electron transfer between the modified GOD and the CP matrix, was observed on the differential pulse voltammograms. The oxidation current of the CP/PEG-GOD electrode was increased by addition of glucose to a solution at the applied potential of 0.10 V versus Ag/AgCl and was proportional to the glucose concentration up to 50 mM. CP/PEG-GOD was stable for a week, which was not improved by the modification of enzyme.

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页码：166 / 168

页数：3

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