DIRECT AND EFFICIENT PLANT-REGENERATION FROM LEAF EXPLANTS OF SOLANUM-TUBEROSUM L CV BINTJE

被引：16

作者：

YADAV, NR ^{[1
]}

STICKLEN, MB ^{[1
]}

机构：

[1] MICHIGAN STATE UNIV, DEPT ENTOMOL, E LANSING, MI 48824 USA

来源：

PLANT CELL REPORTS | 1995年 / 14卷 / 10期

关键词：

BINTJE; POTATO; PLANT REGENERATION; LEAF EXPLANTS; ZEATIN; ZEATIN RIBOSIDE;

D O I：

10.1007/BF00232730

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A two-step procedure was used for plant regeneration from in vitro grown leaf strips (2-3 mm wide) of cv. Bintje. Step I medium was designed with 2,4-dichlorophenoxycetic acid (2,4-D) at 0.0 or 9.0 mu M, in combination with 2.28 mu M kinetin (K), benzyl adenine (BA), zeatin (Z) or zeatin riboside (ZR). Step II media were 2,4-D-free media containing 5.78 mu M gibberellic acid (GA3) and growth regulators similar to those of step I media. Leaf explants cultured in medium I containing zeatin riboside or zeatin for 6 days and then subcultured in medium II containing zeatin riboside produced numerous shoots without callus formation. Zeatin riboside containing step I and II media caused shoot regeneration in a high number (97.5+/-2.2) of explants. Approximately, 33.7+/-8.4 shoots were regenerated from each leaf explant.

引用

页码：645 / 647

页数：3

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