A HISTOCHEMICAL-STUDY OF CHANGES OBSERVED IN THE MOUSE DIAPHRAGM AFTER ORGANOPHOSPHATE POISONING

被引：26

作者：

BRIGHT, JE

INNS, RH

TUCKWELL, NJ

GRIFFITHS, GD

MARRS, TC

机构：

[1] CDE Porton Down, Salisbury

来源：

HUMAN & EXPERIMENTAL TOXICOLOGY | 1991年 / 10卷 / 01期

关键词：

D O I：

10.1177/096032719101000102

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

A sublethal dose of sarin (GB, isopropyl methylphosphonofluoridate) was administered to mice. The animals were killed up to 28 d after dosing and frozen sections were made of the excised diaghragms which were stained using haematoxylin and eosin and a modified Gomori trichrome method. Muscle fibre degeneration and mononuclear infiltration were seen, notably at 24 h and 3 d. A number of histochemical procedures were carried out, including the GBHA procedure for ionized calcium. Calcium accumulation, seen at 4 h, was the earliest abnormality observed. All changes were rapidly regressing by 5 d and histological appearances were normal by 14 d. It was concluded that sarin produced myopathic changes preceded by calcium accumulation.

引用

页码：9 / 14

页数：6

共 27 条

[11]

DUNCAN CJ, 1978, EXPERIENTIA, V43, P1531

[12] IDENTIFICATION OF A NOVEL TYPE-2 FIBER POPULATION IN MAMMALIAN SKELETAL-MUSCLE BY COMBINED USE OF HISTOCHEMICAL MYOSIN ATPASE AND ANTIMYOSIN MONOCLONAL-ANTIBODIES [J].