BRANCHED-CHAIN-AMINO-ACID BIOSYNTHESIS IN PLANTS - MOLECULAR-CLONING AND CHARACTERIZATION OF THE GENE ENCODING ACETOHYDROXY ACID ISOMEROREDUCTASE (KETOL-ACID REDUCTOISOMERASE) FROM ARABIDOPSIS-THALIANA (THALE CRESS)

被引：18

作者：

DUMAS, R ^{[1
]}

CURIEN, G ^{[1
]}

DEROSE, RT ^{[1
]}

DOUCE, R ^{[1
]}

机构：

[1] RHONE POULENC AGROCHIM, SERV BIOL MOLEC & BIOCHIM CELLULAIRE, F-69263 LYON 09, FRANCE

来源：

BIOCHEMICAL JOURNAL | 1993年 / 294卷

关键词：

D O I：

10.1042/bj2940821

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Towards the goal of gaining a better understanding of the molecular mechanisms controlling branched-chain-amino-acid biosynthesis in plants, we have isolated, sequenced and characterized a gene encoding acetohydroxy acid isomeroreductase (ketol-acid reductoisomerase) from Arabidopsis thaliana (thale cress). Comparison between the acetohydroxy acid isomeroreductase cDNA and the genomic sequence has allowed us to determine the exon structure of the coding region. The isolated acetohydroxy acid isomeroreductase gene is distributed over approx. 4.5 kbp and contains nine introns (79-347 bp). The transcriptional start site was found to be 52 bp upstream of the translational initiation site. Southern-blot analysis of A. thaliana genomic DNA shows that the acetohydroxy acid isomeroreductase is encoded by a single-copy gene.

引用

页码：821 / 828

页数：8

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