SEPARATION OF OVERLAPPING SPECTRA FROM EVOLVING SYSTEMS USING FACTOR-ANALYSIS .2. AMPHOTERICIN-B IN AQUEOUS PROPANOL AND IN AQUEOUS LAUROYL SUCROSE

被引：23

作者：

CHAPADOS, C

BARWICZ, J

GRUDA, I

机构：

[1] Departement de chimie-biologie Universite du Quebec a Trois-Rivieres, Trois-Rivières

来源：

BIOPHYSICAL CHEMISTRY | 1994年 / 51卷 / 01期

关键词：

FACTOR ANALYSIS; EIGENSPECTRA; REAL SPECTRA; CONSTRAINTS; ABSORPTION SPECTROSCOPY; AMPHOTERICIN B; LAUROYL SUCROSE; AGGREGATION; MONOMER; OLIGOMER;

D O I：

10.1016/0301-4622(94)00030-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A method based on factor analysis is used to separate the spectra of individual species from the experimental absorption spectra obtained from a fixed amount of amphotericin B dissolved in aqueous solutions. In aqueous propanol solutions and in aqueous lauroyl sucrose solutions, five species are identified: two monomers and three aggregates. Although the spectra of the different species in the two media are similar, the intensity signatures of the two systems are quite different and serve to characterize them.

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页码：71 / 80

页数：10

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