SIMPLE CATION-EXCHANGE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OPTIMIZED TO THE MEASUREMENT OF METABOLITES IN THE EFFLUENTS FROM PERFUSED RAT LIVERS USING REFRACTIVE-INDEX AND ULTRAVIOLET DETECTORS

被引：21

作者：

MASSON, S ^{[1
]}

SCIAKY, M ^{[1
]}

DESMOULIN, F ^{[1
]}

FONTANARAVA, E ^{[1
]}

COZZONE, PJ ^{[1
]}

机构：

[1] FAC MED MARSEILLE,CTR RESONANCE MAGENT BIOL & MED,CNRS,URA 1186,F-13005 MARSEILLE,FRANCE

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1991年 / 563卷 / 02期

关键词：

D O I：

10.1016/0378-4347(91)80031-7

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A high-performance liquid chromatographic method designed to analyse effluents from perfused organs is described. In the case of rat liver, compounds released by the liver are readily separated and quantitated, using a strong cation exchanger (Aminex HPX 87H), two detectors connected in series (ultra-violet detector at 210 nm and refractive index detector), and by optimizing the concentration of sulphuric acid in the mobile phase. Chromatographic conditions described in the present work enable the quantitation, in a single run, of metabolites derived from the tricarboxylic acid cycle, glycolysis, ketogenesis, adenine nucleotides catabolism and ethanol oxidation. The advantage of this method stems from its ease of implementation, sensitivity and flexibility.

引用

页码：231 / 242

页数：12

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