INTER-RELATEDNESS OF SOME ISOENZYMES OF CYTOCHROME-P-450 FROM RAT, RABBIT AND HUMAN, DETERMINED WITH MONOCLONAL-ANTIBODIES

被引：31

作者：

SESARDIC, D

BOOBIS, AR

MCQUADE, J

BAKER, S

LOCK, EA

ELCOMBE, CR

ROBSON, RT

HAYWARD, C

DAVIES, DS

机构：

[1] ROYAL POSTGRAD MED SCH, DEPT CLIN PHARMACOL, LONDON W12 0HS, ENGLAND

[2] ICI PLC, CENT TOXICOL LAB, MACCLESFIELD SK10 4TG, CHESHIRE, ENGLAND

[3] ICI PLC, CORP BIOSCI GRP, RUNCORN WA7 4QE, CHESHIRE, ENGLAND

来源：

BIOCHEMICAL JOURNAL | 1986年 / 236卷 / 02期

关键词：

D O I：

10.1042/bj2360569

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. Monoclonal antibodies have been raised to rat liver cytochromes P-450 b and c, and rabbit liver cytochrome P-450 form 4. A total of six antibodies have been studied. 2. Each antibody reacted strongly both with its homologous antigen and with microsomal fractions selectively enriched with the antigen by treatment of animals with inducing compounds. However, several of the antibodies showed cross-reactivity, either within or between species. 3. A combination of enzyme-linked immunosorbent assay, immunoadsorption, Western blotting and competitive radioimmunoassay revealed that each of the antibodies reacted with a different epitope. 4. Proteolytic digestion of antigen followed by Western blotting of the peptide fragments enabled antibodies, otherwise identical in their reactivity, to be distinguished. 5. It is concluded that complex structural relationships exist amongst the different isoenzymes of cytochrome P-450 and that epitope mapping will help in characterizing both animal and human cytochromes P-450.

引用

页码：569 / 577

页数：9

共 31 条

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