HMG BOX-4 IS THE PRINCIPAL DETERMINANT OF SPECIES-SPECIFICITY IN THE RNA-POLYMERASE-I TRANSCRIPTION FACTOR UBF

被引:19
作者
CAIRNS, C [1 ]
MCSTAY, B [1 ]
机构
[1] UNIV DUNDEE,NINEWELLS HOSP & MED SCH,BIOMED RES CTR,DUNDEE DD1 9SY,SCOTLAND
基金
英国惠康基金;
关键词
D O I
10.1093/nar/23.22.4583
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Transcription of ribosomal genes requires, in addition to RNA polymerase I, the transacting factors UBF and Rib1 in Xenopus or SL1 in humans. RNA polymerase I transcription is remarkably species specific. Between closely related species SL1 is the sole determinant of this specificity. Between more distantly related species, however, UBF is also a component of this species specificity. Xenopus UBF cannot function in human RNA polymerase I transcription and human UBF cannot function in Xenopus RNA polymerase I transcription. Xenopus and human UBFs are remarkably similar at the amino acid sequence level, both containing multiple HMG box DNA binding motifs. The only major difference between xUBF and hUBF is the lack of a HMG box 4 equivalent in xUBF. Utilizing a series of hybrid UBF molecules we have identified HMG box 4 as the principal determinant of species specificity. Addition of human HMG box 4 to xUBF converts it to a form that functions in human RNA polymerase I transcription, Deletion of HMG box 4 from hUBF converts it to a form that functions in Xenopus RNA polymerase I transcription. Furthermore, mutations within Xenopus UBF demonstrate that UBF requires a precise arrangement and number of HMG boxes to function in RNA polymerase I transcription.
引用
收藏
页码:4583 / 4590
页数:8
相关论文
共 36 条
  • [1] HETEROGENEITY IN THE XENOPUS RIBOSOMAL TRANSCRIPTION FACTOR XUBF HAS A MOLECULAR-BASIS DISTINCT FROM THAT IN MAMMALS
    BACHVAROV, D
    NORMANDEAU, M
    MOSS, T
    [J]. FEBS LETTERS, 1991, 288 (1-2) : 55 - 59
  • [2] THE RNA POLYMERASE-I TRANSCRIPTION FACTOR XUBF CONTAINS 5 TANDEMLY REPEATED HMG HOMOLOGY BOXES
    BACHVAROV, D
    MOSS, T
    [J]. NUCLEIC ACIDS RESEARCH, 1991, 19 (09) : 2331 - 2335
  • [3] BAZETT JD, 1994, SCIENCE, V264, P1134
  • [4] MOLECULAR MECHANISMS GOVERNING SPECIES-SPECIFIC TRANSCRIPTION OF RIBOSOMAL-RNA
    BELL, SP
    PIKAARD, CS
    REEDER, RH
    TJIAN, R
    [J]. CELL, 1989, 59 (03) : 489 - 497
  • [5] FUNCTIONAL COOPERATIVITY BETWEEN TRANSCRIPTION FACTOR-UBF1 AND FACTOR-SL1 MEDIATES HUMAN RIBOSOMAL-RNA SYNTHESIS
    BELL, SP
    LEARNED, RM
    JANTZEN, HM
    TJIAN, R
    [J]. SCIENCE, 1988, 241 (4870) : 1192 - 1197
  • [6] ASSEMBLY OF ALTERNATIVE MULTIPROTEIN COMPLEXES DIRECTS RIBOSOMAL-RNA PROMOTER SELECTIVITY
    BELL, SP
    JANTZEN, HM
    TJIAN, R
    [J]. GENES & DEVELOPMENT, 1990, 4 (06) : 943 - 954
  • [7] RECONSTITUTION OF TRANSCRIPTION FACTOR SL1 - EXCLUSIVE BINDING OF TBP BY SL1 OR TFIID SUBUNITS
    COMAI, L
    ZOMERDIJK, JCBM
    BECKMANN, H
    ZHOU, S
    ADMON, A
    TJIAN, R
    [J]. SCIENCE, 1994, 266 (5193) : 1966 - 1972
  • [8] THE TATA-BINDING PROTEIN AND ASSOCIATED FACTORS ARE INTEGRAL COMPONENTS OF THE RNA POLYMERASE-I TRANSCRIPTION FACTOR, SL1
    COMAI, L
    TANESE, N
    TJIAN, R
    [J]. CELL, 1992, 68 (05) : 965 - 976
  • [9] A TBP-CONTAINING MULTIPROTEIN COMPLEX (TIF-IB) MEDIATES TRANSCRIPTION SPECIFICITY OF MURINE RNA POLYMERASE-I
    EBERHARD, D
    TORA, L
    EGLY, JM
    GRUMMT, I
    [J]. NUCLEIC ACIDS RESEARCH, 1993, 21 (18) : 4180 - 4186
  • [10] DNA-BINDING PROPERTIES OF THE HMG DOMAIN OF THE LYMPHOID-SPECIFIC TRANSCRIPTIONAL REGULATOR LEF-1
    GIESE, K
    AMSTERDAM, A
    GROSSCHEDL, R
    [J]. GENES & DEVELOPMENT, 1991, 5 (12B) : 2567 - 2578