THE SEPARATION OF AFFINITY FLOCCULATED YEAST-CELL DEBRIS USING A PILOT-PLANT SCROLL DECANTER CENTRIFUGE

被引:10
作者
BENTHAM, AC
BONNERJEA, J
ORSBORN, CB
WARD, PN
HOARE, M
机构
[1] SERC Centre for Biochemical Engineering, Department of Chemical and Biochemical Engineering, University College London, London, WC1E 7JE, Torrington Place
关键词
D O I
10.1002/bit.260360410
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The use of a scroll decanter centrifuge for the removal and dewatering of affinity‐flocculated yeast cell debris from a crude homogenate is described. Laboratory shear modulus measurements were used to compare the structure of flocculated and nonflocculated sediments and to indicate the dewatering conditions under which the sediment could be discharged from the centrifuge. The structure of the flocculated sediment was such that a dry beach could be used within the centrifuge while still being able to discharge the solids. The scroll decanter performance for recovery and dewatering of the flocculated homogenate was found to be independent of feed flow rate and differential scroll rate. Eighty‐five percent of the solid material was recovered from the flocculated homogenate while the extent of sediment dewatering resulted in the loss of only 7% of the soluble protein in the sediment. The supernatant clarity matched that achieved by low‐gravity laboratory centrifugation studies. Copyright © 1990 John Wiley & Sons, Inc.
引用
收藏
页码:397 / 401
页数:5
相关论文
共 14 条
[1]  
BELL DJ, 1982, BIOTECHNOL BIOENG, V24, P2219
[2]   PROTEIN PRECIPITATE RECOVERY USING MICROPOROUS MEMBRANES [J].
BENTHAM, AC ;
IRETON, MJ ;
HOARE, M ;
DUNNILL, P .
BIOTECHNOLOGY AND BIOENGINEERING, 1988, 31 (09) :984-994
[3]   AFFINITY FLOCCULATION OF YEAST-CELL DEBRIS BY CARBOHYDRATE-SPECIFIC COMPOUNDS [J].
BONNERJEA, J ;
JACKSON, J ;
HOARE, M ;
DUNNILL, P .
ENZYME AND MICROBIAL TECHNOLOGY, 1988, 10 (06) :357-360
[4]   THE RHEOLOGY OF STRONGLY-FLOCCULATED SUSPENSIONS [J].
BUSCALL, R ;
MCGOWAN, IJ ;
MILLS, PDA ;
STEWART, RF ;
SUTTON, D ;
WHITE, LR ;
YATES, GE .
JOURNAL OF NON-NEWTONIAN FLUID MECHANICS, 1987, 24 (02) :183-202
[5]  
FERRY JD, 1980, VISCOELASTIC PROPERT, P11
[6]   CLARIFICATION OF MECHANICALLY DISRUPTED YEAST SUSPENSIONS BY ROTARY VACUUM PRECOAT FILTRATION [J].
GRAY, PP ;
DUNNILL, P ;
LILLY, MD .
BIOTECHNOLOGY AND BIOENGINEERING, 1973, 15 (02) :309-320
[7]  
Hanisch W., 1986, MEMBRANE SEPARATIONS, P61
[8]  
MILBURN P, IN PRESS ENZYME MICR
[9]  
MILLS PDA, STRUCTURE STRENGTH B
[10]   CHARACTERISTICS OF MECHANICALLY DISRUPTED BAKERS-YEAST IN RELATION TO ITS SEPARATION IN INDUSTRIAL CENTRIFUGES [J].
MOSQUEIRA, FG ;
HIGGINS, JJ ;
DUNNILL, P ;
LILLY, MD .
BIOTECHNOLOGY AND BIOENGINEERING, 1981, 23 (02) :335-343