ELDERBERRY (SAMBUCUS-NIGRA L) SEED PROTEINS INHIBIT PROTEIN-SYNTHESIS AND DISPLAY STRONG IMMUNOREACTIVITY WITH RABBIT POLYCLONAL ANTIBODIES RAISED AGAINST THE TYPE-2 RIBOSOME-INACTIVATING PROTEIN NIGRIN-B

被引：30

作者：

CITORES, L ^{[1
]}

IGLESIAS, R ^{[1
]}

MUNOZ, R ^{[1
]}

FERRERAS, JM ^{[1
]}

JIMENEZ, P ^{[1
]}

GIRBES, T ^{[1
]}

机构：

[1] UNIV VALLADOLID,FAC CIENCIAS,DEPT BIOQUIM & BIOL MOLEC,E-47005 VALLADOLID,SPAIN

来源：

JOURNAL OF EXPERIMENTAL BOTANY | 1994年 / 45卷 / 273期

关键词：

ANTI-NIGRIN B ANTIBODIES; PROTEIN SYNTHESIS; SEEDS; ELDER SEEDS; SAMBUCUS NIGRA;

D O I：

10.1093/jxb/45.4.513

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Affinity chromatography-purified elderberry (Sambucus nigra L.) seed proteins strongly inhibited protein synthesis and displayed the 28S rRNA N-glycosidase activity characteristic of all types of ribosome-inactivating proteins (RIPs). Western blot analysis revealed several proteins that reacted with antibodies raised against the novel non-toxic type 2 ribosome-inactivating protein nigrin b isolated from elder bark, thus indicating the presence of a new type 2 RIP.

引用

页码：513 / 516

页数：4

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