Preparation and evaluation of monolithic molecularly imprinted stationary phase for S-naproxen

被引：18

作者：

Chen, De-Miao ^{[1
,2
]}

Fu, Qiang ^{[1
]}

Du, Wei ^{[1
]}

Sun, Si-Juan ^{[1
]}

Huang, Ping ^{[1
]}

Chang, Chun ^{[1
]}

机构：

[1] Xian Inst Food & Drug Control, Xian 710054, Peoples R China

[2] Xi An Jiao Tong Univ, Sch Med, Fac Pharm, Xian 710061, Peoples R China

来源：

JOURNAL OF PHARMACEUTICAL ANALYSIS | 2011年 / 1卷 / 01期

关键词：

molecularly imprinted polymer; naproxen; in situ technique;

D O I：

10.1016/S2095-1779(11)70005-3

中图分类号：

R9 [药学];

学科分类号：

1007 [药学];

摘要：

An S-naproxen (S-NAP) molecularly imprinted monolithic stationary phase (MIMSP) with specific recognition for S-NAP and naproxen (NAP) was prepared by in situ technique, utilizing 4-vinylpridine (4-VP) as a function monomer, ethylene glycol dimethacrylate (EDMA) as a cross-linking agent, and low-polar solvents (toluene and dodecanol) as porogenic solvents. The selectivity of the polymers for S-NAP and NAP was evaluated by high performance liquid chromatography (HPLC). The binding characteristics were tested by Scatchard analysis. Racemic NAP could be specifically separated to some extent. At the same time, NAP could be separated from ibuprofen under optimized conditions. Scatchard analysis showed that two classes of binding sites existed in the S-NAP-imprinted polymers, with their dissociation constants estimated to be 1. 045 and 5.496 JiM, respectively. The results demonstrate that S-NAP and NAP can be recognized specifically on the obtained MIMSP.

引用

页码：26 / 31

页数：6

共 24 条

[1]

ARSHADY R, 1981, MACROMOL CHEM PHYS, V182, P687

[2]

Solvent versatility of bonded cellulose-derived chiral stationary phases for high-performance liquid chromatography and its consequences in column loadability [J].