ELECTROCHEMICAL DETECTION OF PROTEINS IN HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY USING ONLINE, POSTCOLUMN PHOTOLYSIS

被引：9

作者：

DOU, L

HOLMBERG, A

KRULL, IS

机构：

[1] NORTHEASTERN UNIV,DEPT CHEM,BOSTON,MA 02115

[2] ROYAL INST TECHNOL,DEPT BIOCHEM,S-10044 STOCKHOLM 70,SWEDEN

[3] NORTHEASTERN UNIV,BARNETT INST,BOSTON,MA 02115

来源：

ANALYTICAL BIOCHEMISTRY | 1991年 / 197卷 / 02期

关键词：

D O I：

10.1016/0003-2697(91)90407-K

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Direct detection of proteins in high-performance liquid chromatography electrochemistry (LCEC) is difficult. By using on-line, postcolumn photolysis, proteins now can be detected by LCEC at microgram per milliliter levels. The compatibilities of size exclusion chromatography (SEC), reversed-phase chromatography (RPC), ion-exchange chromatography (IEC), and hydrophobic interaction chromatography (HIC) with photolysis-electrochemical detection is described for proteins together with the analytical figures of merit. Inherent from the advantages of electrochemical detection, the method is sensitive and selective. © 1991.

引用

页码：377 / 383

页数：7

共 25 条

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[2]

BRANDENBURG D, 1990, INSULIN

[3]

DOU L, 1990, Biochromatography, V5, P74

[4] POSTCOLUMN DETECTION BY ONLINE PHOTOLYSIS ELECTROCHEMISTRY IN LIQUID-CHROMATOGRAPHY - APPLICATION TO THE DETERMINATION OF PHENYLALANINE IN HUMAN URINE [J].