SUBUNIT COMPOSITION OF HUMAN LIVER ALPHA-L-FUCOSIDASE

被引：12

作者：

ALHADEFF, JA

ANDREWSSMITH, GL

机构：

来源：

BIOCHEMICAL JOURNAL | 1979年 / 177卷 / 02期

关键词：

D O I：

10.1042/bj1770753

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The subunit composition of human liver alpha-L-fucosidase was studied by using three different procedures for treating and electrophoresing the purified enzyme. Although the presence on only one subunit was found by conventional procedures using sodium dodecyl sulphate and urea, two non-identical subunits with mol. wts. of 59 000 and 54 000 were found when the alpha-L-fucosidase was reduced and S-carboxymethylated with iodoacetate followed by electrophoresis on polyacrylamide gels containing 0.1% sodium dodecyl sulphate and 8.0 M-urea.

引用

页码：753 / 756

页数：4

共 28 条

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