CA-2+-CALMODULIN-DEPENDENT PROTEIN KINASE-II PHOSPHORYLATES VARIOUS TYPES OF NONEPITHELIAL INTERMEDIATE FILAMENT PROTEINS

被引：48

作者：

TOKUI, T

YAMAUCHI, T

YANO, T

NISHI, Y

KUSAGAWA, M

YATANI, R

INAGAKI, M

机构：

[1] AICHI CANC CTR,RES INST,EXPTL RADIOL LAB,CHIKUSA KU,NAGOYA,AICHI 464,JAPAN

[2] TOKYO METROPOLITAN INST NEUROSCI,DEPT NEUROCHEM,FUCHU,TOKYO 183,JAPAN

[3] LIFE INFORMAT ANAL CTR,KOMAKI,AICHI 485,JAPAN

[4] MIE UNIV,SCH MED,DEPT THORAC & CARDIOVASC SURG,TSU,MIE 514,JAPAN

[5] MIE UNIV,SCH MED,DEPT PATHOL,TSU,MIE 514,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 169卷 / 03期

基金：

日本科学技术振兴机构;

关键词：

D O I：

10.1016/0006-291X(90)91977-Z

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have investigated the actions of Ca2+•calmodulin (CaM)-dependent protein kinase II on various types of non-epithelial intermediate filament proteins, vimentin, desmin, glial fibrillary acidic protein (GFAP) and neurofilament triplet proteins. Most of these filament proteins could serve as substrates. The effects of phosphorylation on the filamentous structure of vimentin were investigated in sedimentation experiments and by using electron microscopy. The amount of unassembled vimentin increased linearly with increased phosphorylation. However, the extent of the effect of phosphorylation on the potential to polymerize was also affected by the MgCl2 concentration, under conditions for reassembly. The actions of Ca2+•CaM-dependent protein kinase II on non-epithelial intermediate filaments under physiological conditions are given attention. © 1990.

引用

页码：896 / 904

页数：9

共 30 条

[1]

Beavo J A, 1974, Methods Enzymol, V38, P299

[2] CYCLIC AMP-DEPENDENT PROTEIN KINASE-INDUCED VIMENTIN FILAMENT DISASSEMBLY INVOLVES MODIFICATION OF THE N-TERMINAL DOMAIN OF INTERMEDIATE FILAMENT SUBUNITS [J].