IDENTIFICATION OF FAD, FMN, AND RIBOFLAVIN IN THE RETINA BY MICROEXTRACTION AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：41

作者：

BATEY, DW ^{[1
]}

ECKHERT, CD ^{[1
]}

机构：

[1] UNIV CALIF LOS ANGELES,CTR HLTH SCI,DIV NUTR SCI,ROOM 76065,LOS ANGELES,CA 90024

来源：

ANALYTICAL BIOCHEMISTRY | 1990年 / 188卷 / 01期

关键词：

D O I：

10.1016/0003-2697(90)90546-L

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The presence of flavins in the retina has been known for some time. However, the small size of the tissue has made it difficult to quantify the levels of the individual flavins, riboflavin (RB), FMN, and FAD without pooling large numbers of retinas. A procedure to extract and quantitate RB, FMN, and FAD in retinal tissue from as few as four rat retinas has been developed. The procedure resolves these three classes of flavins and provides a recovery near 100%. For the analysis, HPLC using a reverse-phase column with cyclohexyl functional groups was coupled to a fluorescence detector. The microextraction-HPLC procedure was reproducible for the quantitative analysis of flavins in the retina and equally applicable for analysis of flavins in liver and plasma. © 1990.

引用

页码：164 / 167

页数：4