A RADIATION HYBRID MAP OF THE BRCA1 REGION OF CHROMOSOME-17Q12-Q21

被引：53

作者：

ABEL, KJ

BOEHNKE, M

PRAHALAD, M

HO, P

FLEJTER, WL

WATKINS, M

VANDERSTOEP, J

CHANDRASEKHARAPPA, SC

COLLINS, FS

GLOVER, TW

WEBER, BL

机构：

[1] UNIV MICHIGAN, CTR GENOME, DEPT BIOSTAT, ANN ARBOR, MI 48109 USA

[2] UNIV MICHIGAN, CTR GENOME, DEPT HUMAN GENET, ANN ARBOR, MI 48109 USA

[3] UNIV MICHIGAN, CTR GENOME, DEPT PEDIAT, ANN ARBOR, MI 48109 USA

[4] UNIV MICHIGAN, CTR GENOME, HOWARD HUGHES MED INST, ANN ARBOR, MI 48109 USA

来源：

GENOMICS | 1993年 / 17卷 / 03期

关键词：

D O I：

10.1006/geno.1993.1383

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The chromosomal region 17q12-q21 contains a gene (BRCA1) conferring susceptibility to early-onset familial breast and ovarian cancer. An 8000-rad radiation-reduced hybrid (RH) panel was constructed to provide a resource for long-range mapping of this region. A large fraction of the hybrids (∼90%) retained detectable human chromosome 17 sequences. The complete panel of 76 hybrids was scored for the presence or absence of 22 markers from this chromosomal region, including 14 cloned genes, seven microsatellite repeats, and one anonymous DNA segment. Statistical analysis of the marker retention data employing multipoint methods provided both comprehensive and framework maps of this chromosomal region, including distance estimates between adjacent markers. The comprehensive RH map includes 17 loci and spans 179 cRays(8000). Likelihood rations of at least 1000:1 support the 10-locus framework order: cen-D17S250-ERBB2-(THRIA1, TOP2A)-D17S855-PPY-DI7S190-MTBT1-GP3A-BTR-D17S588-tel. The order obtained from RH mapping, when used in conjunction with other methods, will be useful in linkage analysis of breast cancer families and will facilitate the development of a physical map of this region. © 1993 Academic Press, Inc.

引用

页码：632 / 641

页数：10

共 42 条

[11] THE HUMAN NME2 GENE LIES WITHIN 18KB OF NMEI IN CHROMOSOME-17
CHANDRASEKHARAPPA, SC
GROSS, LA
KING, SE
COLLINS, FS
[J]. GENES CHROMOSOMES & CANCER, 1993, 6 (04) : 245 - 248
[12] CLAUS EB, 1991, AM J HUM GENET, V48, P232
[13] AGE AT ONSET AS AN INDICATOR OF FAMILIAL RISK OF BREAST-CANCER
CLAUS, EB
RISCH, NJ
THOMPSON, D
[J]. AMERICAN JOURNAL OF EPIDEMIOLOGY, 1990, 131 (06) : 961 - 972
[14] RADIATION HYBRID MAPPING - A SOMATIC-CELL GENETIC METHOD FOR CONSTRUCTING HIGH-RESOLUTION MAPS OF MAMMALIAN CHROMOSOMES
COX, DR
BURMEISTER, M
PRICE, ER
KIM, S
MYERS, RM
[J]. SCIENCE, 1990, 250 (4978) : 245 - 250
[15] EASTON DF, 1993, AM J HUM GENET, V52, P678
[16] FAIN PR, 1991, CYTOGENET CELL GENET, V57, P66
[17] FAIN PR, 1992, CYTOGENET CELL GENET, V60, P178
[18] FLETJER WL, 1993, GENOMICS, V17, P624
[19] A PANEL OF IRRADIATION-REDUCED HYBRIDS SELECTIVELY RETAINING HUMAN CHROMOSOME-11P13 - THEIR STRUCTURE AND USE TO PURIFY THE WAGR GENE-COMPLEX
GLASER, T
ROSE, E
MORSE, H
HOUSMAN, D
JONES, C
[J]. GENOMICS, 1990, 6 (01) : 48 - 64
[20] CLONING AND SEQUENCING OF THE CDNA-ENCODING A CORE PROTEIN OF THE PAIRED HELICAL FILAMENT OF ALZHEIMER-DISEASE - IDENTIFICATION AS THE MICROTUBULE-ASSOCIATED PROTEIN TAU
GOEDERT, M
WISCHIK, CM
CROWTHER, RA
WALKER, JE
KLUG, A
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (11) : 4051 - 4055

← 1 2 3 4 5 →