SOFT-X-RAY MAGNETIC CIRCULAR-DICHROISM - A PROBE FOR STUDYING PARAMAGNETIC BIOINORGANIC SYSTEMS

被引：28

作者：

VANELP, J

GEORGE, SJ

CHEN, J

PENG, G

CHEN, CT

TJENG, LH

MEIGS, G

LIN, HJ

ZHOU, ZH

ADAMS, MWW

SEARLE, BG

CRAMER, SP

机构：

[1] LAWRENCE BERKELEY LAB, DIV ENERGY & ENVIRONM, BERKELEY, CA 94720 USA

[2] UNIV GEORGIA, CTR METALLOENZYME STUDIES, ATHENS, GA 30602 USA

[3] UNIV GRONINGEN, CTR MAT SCI, DEPT APPL & SOLID STATE PHYS, 9747 AG GRONINGEN, NETHERLANDS

[4] AT&T BELL LABS, MURRAY HILL, NJ 07974 USA

[5] UNIV CALIF DAVIS, DEPT APPL SCI, DAVIS, CA 95616 USA

[6] UNIV GEORGIA, DEPT BIOCHEM, ATHENS, GA 30602 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1993年 / 90卷 / 20期

关键词：

X-RAY ABSORPTION SPECTROSCOPY; PYROCOCCUS-FURIOSUS;

D O I：

10.1073/pnas.90.20.9664

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Soft x-ray magnetic circular dichroism was used to study a paramagnetic bioinorganic system. We measured the Fe L edges of Pyrococcus furiosus rubredoxin, using circularly polarized synchrotron radiation, a split-coil superconducting magnet, low sample temperatures, and fluorescence detection. The observed dichroism effect is strong (30%) and in general agreement with the calculation. The method is element- and oxidation state-specific, and the data can be interpreted by established theoretical procedures. Soft x-ray magnetic circular dichroism demonstrates enormous potential as a probe for studying paramagnetic systems in biology, chemistry, and material science.

引用

页码：9664 / 9667

页数：4

共 24 条

[1] DETERMINANTS OF PROTEIN HYPERTHERMOSTABILITY - PURIFICATION AND AMINO-ACID-SEQUENCE OF RUBREDOXIN FROM THE HYPERTHERMOPHILIC ARCHAEBACTERIUM PYROCOCCUS-FURIOSUS AND SECONDARY STRUCTURE OF THE ZINC ADDUCT BY NMR [J].