LIPOLYTIC ACTION OF FRUCTOSE-1-6-DIPHOSPHATE

In an attempt to elucidate the mechanism of the lipolytic action of glucose, the effect on lipolysis of (a) inhibitors of aerobic processes, (b) stimulants of the shunt pathway, and (c) glycolytic intermediates was studied in an in vitro system using intact rat adipose tissue and homogenates. (a) completely abolished the lipolytic effect of glucose and (b) had no effect. Most of (c) had a small lipolytic effect comparable to that of glucose with the exception of fructose-1-6-diphosphate (F-1-6-DiP) which markedly stimulated lipolysis in both intact tissue and homogenates. This effect of F-1-6-DiP is not due to its generating L-α-glycerophosphate, and is probably a specific activating effect of F-1-6-DiP on the lipolytic (enzyme) system of adipose tissue. A comparison between the lipolytic action of adrenaline and of F-1-6-DiP has suggested that the mechanism of action of these two agents on lipolysis is not identical. © 1968.