N-METHYL-N'-NITRO-N-NITROSOGUANIDINE MUTAGENESIS AND LETHALITY IN AEROBACTER (KLEBSIELLA) AEROGENES

被引：10

作者：

BEACH, C

DEAN, ACR

机构：

[1] Physical Chemistry Laboratory, Oxford University

来源：

MOLECULAR AND GENERAL GENETICS | 1969年 / 104卷 / 04期

关键词：

D O I：

10.1007/BF00334237

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. A survey is made of the induction of auxotrophy in Aerobacter (Klebsiella) aerogenes by MNNG (N-methyl-N′-nitro-N-nitrosoguanidien). 2. Of a variety of simple media of pH 6.0, the yield of auxotrophs was highest on treatment in tris-maleic acid buffer but, in general, the lethal action increased the more closely the medium approached to a complete (minimal) growth medium. In nutrient broth (pH 7.5) the low activity of MNNG was potentiated by the addition of nucleotide bases; adenine, guanine and cytosine increased mutagenesis considerably, and hypoxanthine the lethality. 3. Possible mechanisms for the observed effects are discussed. © 1969 Springer-Verlag.

引用

页码：378 / &

共 11 条

[1] OPTIMAL CONDITIONS FOR MUTAGENESIS BY N-METHYL-N'-NITRO-N-NITROSOGUANIDINE IN ESCHERICHIA COLI K12 [J].

ADELBERG, EA ;

MANDEL, M ;

CHEN, GCC .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1965, 18 (5-6) :788-&

[2] DIAZOMETHANE AS ACTIVE AGENT IN NITROSOGUANIDINE MUTAGENESIS AND LETHALITY [J].

CERDAOLM.E ;

HANAWALT, PC .

MOLECULAR AND GENERAL GENETICS, 1968, 101 (03) :191-&

[3] REACTION OF N-METHYL-N'-NITRO-N-NITROSOGUANIDINE WITH DEOXYRIBONUCLEIC ACID [J].

CRADDOCK, VM .

BIOCHEMICAL JOURNAL, 1968, 106 (04) :921-&

[4] STUDIES ON ACTION OF DIAZOMETHANE ON DEOXYRIBONUCLEIC ACID - ACTION OF DIAZOMETHANE ON DEOXYRIBONUCLEOSIDES [J].

FRIEDMAN, OM ;

MAHAPATR.GN ;

DASH, B ;

STEVENSO.R .

BIOCHIMICA ET BIOPHYSICA ACTA, 1965, 103 (02) :286-&

[5]

GREENBERG J, 1961, ANTIBIOT CHEMOTHER, V11, P52

[6] THE REACTION OF AMINES WITH N-METHYL-N-NITROSO-N'-NITROGUANIDINE [J].

HENRY, RA .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1950, 72 (07) :3287-3289

[7] EFFECT OF SOME TREATMENT CONDITIONS ON RADIOMIMETIC ACTIVITY OF 1-METHYL-3-NITRO-1-NITROSOGUANIDINE IN PLANTS [J].

KAUL, BL .

MUTATION RESEARCH, 1969, 7 (01) :43-&

[8] METHYLATION OF DEOXYRIBONUCLEIC ACID BY DIAZOMETHANE [J].

KRIEK, E ;

EMMELOT, P .

BIOCHIMICA ET BIOPHYSICA ACTA, 1964, 91 (01) :59-&

[9] METHYLATION OF DNA BY N-METHYL-N-NITROSOURETHANE AND N-METHYL-N-NITROSO-N'-NITROGUANIDINE [J].

LAWLEY, PD .

NATURE, 1968, 218 (5141) :580-&

[10] REACTION OF N-METHYL-N'-NITRO-N-NITROGUANIDINE AND N-METHYL-N-NITROSO-P-TOLUENESULFONAMIDE WITH DNA IN VITRO [J].

MCCALLA, DR .

BIOCHIMICA ET BIOPHYSICA ACTA, 1968, 155 (01) :114-&

← 1 2 →