PURIFICATION AND SUBUNIT DETERMINATION OF THE NICKEL-DEPENDENT STAPHYLOCOCCUS-XYLOSUS UREASE

被引：7

作者：

CHRISTIANS, S

JOSE, J

SCHAFER, U

KALTWASSER, H

机构：

[1] UNIV SAARLAND,FACHRICHTUNG MIKROBIOL,STADTWALD,W-6600 SAARBRUCKEN,GERMANY

[2] ZENTRUM MOLEK BIOL,HEIDELBERG,GERMANY

来源：

FEMS MICROBIOLOGY LETTERS | 1991年 / 80卷 / 2-3期

关键词：

UREASE; ENZYME PURIFICATION; NICKEL; STAPHYLOCOCCUS-XYLOSUS;

D O I：

10.1111/j.1574-6968.1991.tb04674.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The Staphylococcus xylosus C2a urease was purified 655-fold to a maximum specific activity of 1573 U/mg and contained three non-identical subunits (64, 17.8 and 16.3 kDa). The purified urease had a total molecular mass of 300 kDa and contained 3.91 +/- 0.1 g atoms nickel per molecule as determined by atomic absorption spectrometry. The maximum urease activity occurred at pH 7.2 and 65-degrees-C, with a pI between 4 and 5.

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页码：271 / 276

页数：6

相关论文

共 19 条

[1] ESTIMATION OF MOLECULAR WEIGHTS OF PROTEINS BY SEPHADEX GEL-FILTRATION [J].

ANDREWS, P .

BIOCHEMICAL JOURNAL, 1964, 91 (02) :222-+

[2]

CHANEY AL, 1962, CLIN CHEM, V8, P130

[3] NICKEL-CONTENT OF UREASE FROM BACILLUS-PASTEURII [J].

CHRISTIANS, S ;

KALTWASSER, H .

ARCHIVES OF MICROBIOLOGY, 1986, 145 (01) :51-55

[4] PROPERTIES OF UREASE FROM UREAPLASMA-UREALYTICUM - KINETICS, MOLECULAR-WEIGHT, AND DEMONSTRATION OF MULTIPLE ENZYME ISOELECTRIC POINT FORMS [J].

ENG, H ;

ROBERTSON, JA ;

STEMKE, GW .

CANADIAN JOURNAL OF MICROBIOLOGY, 1986, 32 (06) :487-493

[5]

FISHBEIN WN, 1969, 5TH INT S CHROM EL, P238

[6] STAPHYLOCOCCUS-SAPROPHYTICUS UREASE - CHARACTERIZATION AND CONTRIBUTION TO UROPATHOGENICITY IN UNOBSTRUCTED URINARY-TRACT INFECTION OF RATS [J].

GATERMANN, S ;

JOHN, J ;

MARRE, R .

INFECTION AND IMMUNITY, 1989, 57 (01) :110-116

[7] CLONING AND EXPRESSION OF VARIOUS STAPHYLOCOCCAL GENES ENCODING UREASE IN STAPHYLOCOCCUS-CARNOSUS [J].

JOSE, J ;

CHRISTIANS, S ;

ROSENSTEIN, R ;

GOTZ, F ;

KALTWASSER, H .

FEMS MICROBIOLOGY LETTERS, 1991, 80 (2-3) :277-282

[8]

KAKIMOTO S, 1990, APPL MICROBIOL BIOT, V32, P538

[9] PURIFICATION AND CHARACTERIZATION OF ACID UREASE FROM LACTOBACILLUS-REUTERI [J].

KAKIMOTO, S ;

SUMINO, Y ;

AKIYAMA, S ;

NAKAO, Y .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1989, 53 (04) :1119-1125

[10] NADH-DEPENDENT COUPLED ENZYME ASSAY FOR UREASE AND OTHER AMMONIA-PRODUCING SYSTEMS [J].

KALTWASS.H ;

SCHLEGEL, HG .

ANALYTICAL BIOCHEMISTRY, 1966, 16 (01) :132-&