BINDING OF 5-HYDROXYTRYPTAMINE ANALOGS BY ISOLATED SPISULA SPERM MEMBRANE

被引：15

作者：

BANDIVDEKAR, AH

SEGAL, SJ

KOIDE, SS

机构：

[1] POPULAT COUNCIL,CTR BIOMED RES,1230 YORK AVE,NEW YORK,NY 10021

[2] INDIAN COUNCIL MED RES,INST RES REPROD,BOMBAY 400012,INDIA

[3] ROCKEFELLER UNIV,POPULAT SCI,NEW YORK,NY 10021

来源：

INVERTEBRATE REPRODUCTION & DEVELOPMENT | 1992年 / 21卷 / 01期

关键词：

SEROTONIN; SPISULA SPERM; SPERM MOTILITY; 5-HT RECEPTORS;

D O I：

10.1080/07924259.1992.9672218

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Serotonin receptors were identified in Spisula sperm membranes by radioligand binding assay. Sperm membranes were prepared by nitrogen cavitation and by density gradient ultracentrifugation. Specific binding of [H-3]5-HT to the receptors of isolated sperm membranes was determined. The dissociation constant was 2.7 nM and the maximum binding capacity was 11.25 pmoles/mg of protein. The order of decreasing potency of 5-HT agonists in blocking [H-3]5-HT binding to the sperm membrane was as follows: 2-methyl 5-HT > 8-OH-DPAT > 5-HT > 5-CT > alpha-methyl-5-HT; and that of 5-HT antagonists was: ICS-205-930 > BMY-7378 > mianserin > methysergide. These findings suggest that the 5-HT receptors in Spisula sperm membranes contain sites that interact with 5-HT3 and 5-HT1 analogs.

引用

页码：43 / 46

页数：4

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