CHARACTERIZATION OF THE GENE FAMILY ENCODING A HOST-PROTECTIVE ANTIGEN OF THE TAPEWORM TAENIA-OVIS

被引:33
作者
WATERKEYN, JG
LIGHTOWLERS, MW
COPPEL, R
COWMAN, AF
机构
[1] MONASH UNIV, DEPT MICROBIOL, CLAYTON, VIC 3168, AUSTRALIA
[2] WALTER & ELIZA HALL INST MED RES, MELBOURNE, VIC 3050, AUSTRALIA
关键词
TAENIA OVIS; MULTIGENE FAMILY; GENOMIC STRUCTURE; VACCINE ANTIGEN; ALLELE;
D O I
10.1016/0166-6851(94)00104-U
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Genomic structure has been determined for a gene encoding a host-protective antigen of the parasitic platyhelminth Taenia ovis. An incomplete cDNA, known as 45W, encodes the protective antigen. Southern hybridisation experiments using 45W cDNA as a probe, revealed that the 45W gene was a member of a multigene family. Differential Southern hybridisation and rapid amplification of cDNA end (RACE) experiments were used to characterise the related genes, allowing the full-length coding region of the 45W encoded antigen to be determined. The gene family comprises a minimum of four members per haploid genome with each member showing varying degrees of 5' and 3' homology with respect to the 45W cDNA. A close homologue of the 45W gene, designated 45S, differed from 45W at 11 of 985 nt comprising the full-length mRNA. Sequencing of several independent RACE products for both 45W and 45S identified a cDNA which may be a product of homologous recombination between these genes, suggesting that the two genes may be alleles. Homologous recombination in genes which encode a host protective antigen such as 45W would provide a mechanism by which antigenic variants could arise.
引用
收藏
页码:123 / 131
页数:9
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