KINETICS OF GROWTH AND METABOLISM OF CLOSTRIDIUM-THERMOSACCHAROLYTICUM CULTURE - ISOLATION AND CHARACTERISTICS OF ITS PLASMIDS

被引：3

作者：

BELOGUROVA, NG ^{[1
]}

MOSOLOVA, TP ^{[1
]}

KALYUZHNYY, SV ^{[1
]}

VARFOLOMEYEV, SD ^{[1
]}

机构：

[1] MV LOMONOSOV STATE UNIV,DEPT CHEM,DIV CHEM ENZYMOL,MOSCOW 119899,USSR

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1991年 / 27卷 / 01期

关键词：

CLOSTRIDIUM-THERMOSACCHAROLYTICUM; GLUCOSE; HYDROGEN; ETHANOL; ACETIC; PROPIONIC AND LACTIC ACIDS; CARBON DIOXIDE; KINETICS; SPECIFIC GROWTH RATE; HALF-SATURATION CONSTANT; INHIBITION CONSTANT; ANTIBIOTIC-RESISTANCE; STREPTOMYCIN; KANAMYCIN; PLASMID; RESTRICTION ANALYSIS;

D O I：

10.1007/BF02921510

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The kinetics of growth and metabolism of Clostridium thermosaccharolyticum DSM 571 has been studied at varying initial pH and glucose concentration. A weak inhibitory effect of excess glucose on the specific growth rate has been shown. The effect of antibiotics of various classes on culture growth and hydrogen evolution has been studied. Streptomycin and kanamycin resistance of this cultuure has been revealed as well as the phenomenon of increased hydrogen production in the presence of the above antibiotics. New plasmids, pNB1 (4.9 kb) and pNB2 (2.0 kb), were isolated from C. thermosaccharolyticum DSM 571. The restriction analysis of pNB1 and pNB2 has been performed.

引用

页码：1 / 8

页数：8

共 19 条

[1]

BEREZIN IV, 1977, BIOKHIMIYA, V9, P1631

[2]

BIRNBOIM HC, 1979, NUCLEIC ACIDS RES, V7, P1513

[3]

FECHINA VA, 1988, 6TH P ALL UN S ENZ E, V1, P155

[4]

Gorina I A, 1980, Prikl Biokhim Mikrobiol, V16, P936

[5] GENETIC-RELATIONSHIP BETWEEN PUB110 AND ANTIBIOTIC-RESISTANT PLASMIDS OBTAINED FROM THERMOPHILIC BACILLI [J].

HOSHINO, T ;

IKEDA, T ;

FURUKAWA, K ;

TOMIZUKA, N .

CANADIAN JOURNAL OF MICROBIOLOGY, 1985, 31 (07) :614-619

[6] ISOLATION AND CHARACTERIZATION OF ANTIBIOTIC-RESISTANCE PLASMIDS FROM THERMOPHILIC BACILLI AND CONSTRUCTION OF DELETION PLASMIDS [J].

IMANAKA, T ;

FUJII, M ;

AIBA, S .

JOURNAL OF BACTERIOLOGY, 1981, 146 (03) :1091-1097

[7] SCREENING FOR PLASMIDS IN THE GENUS CLOSTRIDIUM [J].

LEE, CK ;

DURRE, P ;

HIPPE, H ;

GOTTSCHALK, G .

ARCHIVES OF MICROBIOLOGY, 1987, 148 (02) :107-114

[8]

Maniatis T, 1982, MOL CLONING LABORATO, P89

[9] ENZYMATIC AND NUCLEOTIDE-SEQUENCE STUDIES OF A KANAMYCIN-INACTIVATING ENZYME ENCODED BY A PLASMID FROM THERMOPHILIC BACILLI IN COMPARISON WITH THAT ENCODED BY PLASMID PUB110 [J].

MATSUMURA, M ;

KATAKURA, Y ;

IMANAKA, T ;

AIBA, S .

JOURNAL OF BACTERIOLOGY, 1984, 160 (01) :413-420

[10] Studies on anaerobic bacteria IV. Taxonomy of cultures of a thermophilic species causing "swells" of canned foods [J].

McClung, LS .

JOURNAL OF BACTERIOLOGY, 1935, 29 (02) :189-U3

← 1 2 →